Molecular imprinting : from fundamentals to applications
著者
書誌事項
Molecular imprinting : from fundamentals to applications
Wiley-VCH, c2003
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Molecular imprinting, the polymerization of monomers in the presence of a template molecule which imprints structural information into the resulting polymers, is a scientific field which is rapidly gaining significance for a widening range of applications in biotechnology, biochemistry and pharmaceutical research.
The methods and tools needed to distinguish target molecules from others by means of tailor-made receptors are constantly growing in importance and complexity. This book gives a concise and highly up-to-date overview of the remarkable progress made in this field in the last five years.
The material is comprehensively presented by the authors, giving a thorough insight into fundamentals and applications for researchers in both industry and academy.
目次
Preface
INTRODUCTION
Importance of Receptor Molecules in Advanced Science and Technology
Naturally Ocurring Receptors
Artificial Receptors
Receptors for Practical Applications
Why is the Molecular Imprinting Method so Promising?
FUNDAMENTALS OF MOLECULAR IMPRINTING
Introduction
General Principle of Molecular Imprinting
Covalent Imprinting and Non-covalent Imprinting
Advantages and Disadvantages of Covalent and Non-Covalent Imprinting
History
EXPERIMENTAL METHODS (I) -
PROCEDURES OF MOLECULAR IMPRINTING
Introduction
Reagents and Experimental Procedures
Covalent Imprinting
Non-covalent Imprinting
"Dummy Molecular Imprinting"
EXPERIMENTAL METHODS (II) -
EVALUATION OF IMPRINTING EFFICIENCY
Chromatographic Experiments
Batchwise Guest-Binding Experiments
Determination of Guest-Binding Constants
SPECTROSCOPIC ANATOMY OF MOLECULAR IMPRINTING REACTIONS
Introduction
Structures of Adducts in the Pre-organization Step
Determination of the Binding Constant K for the Formation of Monomer-Template Adduct
Relationship between K Value and Imprinting Efficiency
Structure of the Guest-Binding Site
FLOW-CHART OF A TYPICAL MOLECULAR IMPRINTING
Introduction
Choice of Agents
Polymerization
Packing of Imprinted Polymers into the HPLC Columns
Quantitative Evaluation of Imprinting Efficiency
APPLICATIONS OF MOLECULARLY IMPRINTED POLYMERS
Introduction
Sensor Applications
Signaling Polymers
Molecularly Imprinted Sorbent Assays
Molecularly Imprinted Membranes
Affinity-based Solid-phase Extraction
Molecularly Imprinted Catalysts
RECENT CHALLENGES AND PROGRESS
Introduction
Molecular Imprinting in Water
Use of Two Kinds of Functional Monomers for Cooperative Recognition
Inorganic Gel as the Matrix for Molecular Imprinting
Artificial Enzyme (Molecular Catalyst) by Molecular Imprinting
Catalytic Antibody Prepared by Using Transition-State Analog
CONCLUSIONS AND PROSPECTS
Index
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