Thyroid hormone receptors : methods and protocols
著者
書誌事項
Thyroid hormone receptors : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, v. 202)
Humana Press, c2002
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
A panel of outstanding investigators surveys and explains the major cutting-edge methods used in thryroid receptor (TR) research and explains their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TR in development and knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and in living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for studying the oncogene v-Erba in blood cell differentiation, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver.
目次
Introduction to Thyroid Hormone Receptors
Aria Baniahmad
Null Mutant Mice for Thyroid Hormone Receptors
Karine Gauthier, Denise Aubert, Olivier Chassande, Frederic Flamant, and Jacques Samarut
Transgenic Targeting of a Dominant Negative Corepressor to Liver and Analyses by cDNA Microarray
Xu Feng, Paul Meltzer, and Paul M. Yen
The Role of Thyroid Hormone Receptors in the Heart
Wolfgang H. Dillmann and Bernd R. Gloss
Analysis of Thyroid Hormone-Dependent Genes in the Brain by In Situ Hybridization
Juan Bernal and Ana Guadano-Ferraz.
The v-erbA Oncogene: Assessing Its Differentiation-Blocking Ability Using Normal Chicken Erythrocytic Progenitor Cells,
Olivier Gandrillon
Gene Regulation by Thyroid Hormone Receptors
Helmut Dotzlaw and Aria Baniahmad
Molecular Analysis of Human Resistance to Thyroid Hormone Syndrome
Sunnie M. Yoh and Martin L. Privalsky
Function of Thyroid Hormone Receptors During Amphibian Development
Sashko Damjanovski, Laurent M. Sachs, and Yun-Bo Shi
Transcriptional Regulation by Thyroid Hormone Receptor in Chromatin
Jiemin Wong
Gene Activation by Thyroid Hormone Receptor In Vitro and Purification of the TRAP Coactivator Complex
Joseph D. Fondell
Index
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