Electric field-induced effects on neuronal cell biology accompanying dielectrophoretic trapping
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Bibliographic Information
Electric field-induced effects on neuronal cell biology accompanying dielectrophoretic trapping
（Advances in anatomy, embryology and cell biology, v. 173）
Springer, 2003
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Includes bibliographical references and index
Description and Table of Contents
Description
1. 1 Neuro-Electronic Interfacing 1. 1. 1 Nervous System Communication in the(human) bodyand the interaction with the environment is controlled by the nervous system. It can be divided into a central part, which - cludes the spinal cord, brainstem, cerebellum, and cerebrum, and a peripheral part, which includes all neuronal tissue outside the central part (Martini 2001). The latter provides the interface between the central nervous system and the internal and ext- nal environment of the body. Eye, ear, skin, and muscle sensors provide the nec- sary information. Via primary afferent neurons this information is transmitted to the central nervous system. Conversely,this system provides information to the - tor organs via theefferent fibers. Furthermore, the central nervous system is resp- sible for cognition, learning, and memory. Neurons are cells specialized for receiving information and transmitting signals to other neurons or to effector cells, such as muscles and glands (Levitan 1991). Like all other cells, neurons are enclosed by a cell membrane, which is a double layer of phospholipid molecules.
This bilayer, about 10 nm thick, serves as a barrier that - lows the cell to maintain an internal (cytoplasmic) composition far different from the composition of the extracellular fluid. It contains enzymes, receptors, and an- gens that play central roles in the interaction of thecell with other cells.
Table of Contents
Introduction.- Neuro-electronic interfacing.- Culturing neuronal cells.- Positioning and culturing neuronal cells on a micro-electrode array.- Dielectrophoresis.- Scope of this review.- Dielectrophoretic trapping of neuronal cells.- Theory.-Materials.- Theoretical description of dielectrophoretic trapping.- Experimental description of dielectrophoretic trapping.- Exposing neuronal cells to electric fields.- Theory.- Theoretical investigation of induced membrane potentials of neuronal cells.- Experimental investigation of neuronal membrane breakdown.- Investigating viability of dielectrophoretically trapped neuronal cells.- Viability of neuronal cells trapped at a high frequency.- Viability of neuronal cells trapped at low frequencies.- Recording neuronal activity.- Summary.- References.- Subject index.
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