RNAi : a guide to gene silencing
著者
書誌事項
RNAi : a guide to gene silencing
Cold Spring Harbor Laboratory Press, c2003
- : hardcover
- : pbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: hardcover ISBN 9780879696412
内容説明
The ability of double stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done.
- 巻冊次
-
: pbk ISBN 9780879697044
内容説明
The ability of double-stranded RNA to inhibit the expression of genes with an appropriate sequence can be harnessed to silence target genes in vitro in a new and powerful way. This volume combines reliable RNAi protocols for a variety of species with discussion of strategies for the effective design of experiments using this important new technique that is changing the way experimental science is done.
目次
- Chapter 1. Sense Cosuppression in Plants: Past, Present, and Future Richard A. Jorgensen Chapter 2. Transgene Cosuppression in Animals James A. Birchler, Manika Pal-Bhadra, and Utpal Bhadra Chapter 3. Regulation of the Genome by Double-stranded RNA Marjori Matzke, M. Florian Mette, Tatsuo Kanno, Werner Aufsatz, and Antonius J.M. Matzke Chapter 4. RNAi in Caenorhabditis elegans Ren e9
- F. Ketting, Marcel Tijsterman, and Ronald H.A. Plasterk Chapter 5. Small RNAs, Big Biology: Biochemical Studies of RNA Interference Gregory J. Hannon and Phillip D. Zamore Chapter 6.shRNA-mediated Silencing of Mammalian Gene Expression Michael T. McManus and Douglas S. Conklin Chapter 7. Transposable Elements, RNA Interference, and the Origin of Heterochromatin Rob Martienssen, Tom Volpe, Zach Lippman, Ann-Valerie Gendrel, Catherine Kidner, Pablo Rabinowicz, and Vincent Colot Chapter 8. The Ribonuclease III Superfamily: Forms and Functions in RNA Maturation, Decay, and Gene Silencing Allen W. Nicholson Chapter 9. RNA-dependent RNA Polymerase in Gene Silencing Luyun Huang, John Gledhill, and Craig E. Cameron Chapter 10. Structure and Function of Heterochromatin: Implications for Epigenetic Gene Silencing and Genome Organization Ira M. Hall and Shiv I.S. Grewal Chapter 11. Total Interference: Genome-wide RNAi Screens in C. elegans Andrew G. Fraser and Julie Ahringer Chapter 12. PTGS Approaches to Large Scale Functional Genomics in Plants Tessa M. Burch-Smith, Jennifer L. Miller, and S.P. Dinesh-Kumar Chapter 13. Mammalian RNA Interference Thomas Tuschl Chapter 14. RNAi in Avian Embryos Esther T. Stoeckli Chapter 15. Guide to RNAi in Mouse Oocytes and Preimplantation Embryos Paula Stein and Petr Svoboda Chapter 16. RNAi Technologies in Drosophila Cell Culture Scott M. Hammond Chapter 17. RNAi Applications in Drosophila melanogaster Richard W. Carthew Chapter 18. RNA Interference in Trypanosoma brucei and Other Nonclassical Model Organisms Elisabetta Ullu and Christian Tschudi
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