Plant functional genomics

著者

    • Grotewold, Erich

書誌事項

Plant functional genomics

edited by Erich Grotewold

(Methods in molecular biology / John M. Walker, series editor, v. 236)

Humana Press, c2003

  • e-book

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Functional genomics is a young discipline whose origin can be traced back to the late 1980s and early 1990s, when molecular tools became available to determine the cellular functions of genes. Today, functional genomics is p- ceived as the analysis, often large-scale, that bridges the structure and organi- tion of genomes and the assessment of gene function. The completion in 2000 of the genome sequence of Arabidopsis thaliana has created a number of new and exciting challenges in plant functional genomics. The immediate task for the plant biology community is to establish the functions of the approximately 25,000 genes present in this model plant. One major issue that will remain even after this formidable task is c- pleted is establishing to what degree our understanding of the genome of one model organism, such as the dicot Arabidopsis, provides insight into the or- nization and function of genes in other plants. The genome sequence of rice, completed in 2002 as a result of the synergistic interaction of the private and public sectors, promises to significantly enrich our knowledge of the general organization of plant genomes. However, the tools available to investigate gene function in rice are lagging behind those offered by other model plant systems. Approaches available to investigate gene function become even more limited for plants other than the model systems of Arabidopsis, rice, and maize.

目次

Part I. Finding Genes in Complex Plant Systems An Improved Method for Plant BAC Library Construction Meizhong Luo and Rod A. Wing Constructing Gene-Enriched Plant Genomic Libraries Using Methylation Filtration Technology Pablo D. Rabinowicz RescueMu Protocols for Maize Functional Genomics Manish N. Raizada Precious Cells Contain Precious Information: Strategies and Pitfalls in Expression Analysis from a Few Cells Isabelle M. Henry and Dina F. Mandoli Combined ESTs from Plant-Microbe Interactions: Using GC Counting to Determine the Species of Origin Edgar Huitema, Trudy A. Torto, Allison Styer, and Sophien Kamoun Part II. In Silico Prediction of Plant Gene Function Computer Software to Find Genes in Plant Genomic DNA Ramana V. Davuluri and Michael Q. Zhang Genomic Colinearity as a Tool for Plant Gene Isolation Wusirika Ramakrishna and Jeffrey L. Bennetzen Using Natural Allelic Diversity to Evaluate Gene Function Sherry R. Whitt and Edward S. Buckler, IV Quantitative Trait Locus Analysis as a Gene Discovery Tool Michael D. McMullen Part III. Forward and Reverse Genetic Strategies Transposon Tagging Using Activator (Ac) in Maize Thomas P. Brutnell and Liza J. Conrad T-DNA Mutagenesis in Arabidopsis Jose M. Alonso and Anna N. Stepanova Physical and Chemical Mutagenesis Andrea Kodym and Rownak Afza High-Throughput TILLING for Functional Genomics Bradley J. Till, Trenton Colbert, Rachel Tompa, Linda C. Enns, Christine A. Codomo, Jessica E. Johnson, Steven H. Reynolds, Jorja G. Henikoff, Elizabeth A. Greene, Michael N. Steine, Luca Comai, and Steven Henikoff Gene and Enhancer Traps for Gene Discovery Marcela Rojas-Pierce and Patricia S. Springer High-Throughput TAIL-PCR as a Tool to Identify DNA Flanking Insertions Tatjana Singer and Ellen Burke Custom Knock-Outs with Hairpin RNA-Mediated Gene Silencing Susan Varsha Wesley, Qing Liu, Anna Wielopolska, Geoff Ellacott, Neil Smith, Surinder Singh, and Chris Helliwell Virus-Induced Gene Silencing S. P. Dinesh-Kumar, Radhamani Anandalakshmi, Rajendra Marathe, Michael Schiff, and Yule Liu Exploring the Potential of Plant RNase P as a Functional Genomics Tool Dileep K. Pulukkunat, M. L. Stephen Raj, Debasis Pattanayak, Lien B. Lai, and Venkat Gopalan Maintaining Collections of Mutants for Plant Functional Genomics Randy Scholl, Martin M. Sachs, and Doreen Ware Part IV. Gain of Function Approaches Vector Construction for Gene Overexpression as a Tool to Elucidate Gene Function Alan Lloyd T-DNA Activation Tagging Johan Memelink Part V. Phenotypic Profiling as a Tool to Determine Gene Function Expression Profiling Using cDNA Microarrays Suling Zhao and Wesley B. Bruce Open Architecture Expression Profiling of Plant Transcriptomes and Gene Discovery Using GeneCalling (R) Technology Oswald R. Crasta and Otto Folkerts Proteomics as a Functional Genomics Tool Ulrike Mathesius, Nijat Imin, Siria H. A. Natera, and Barry G. Rolfe Metabolite Profiling as a Functional Genomics Tool Anusha P. Dias, Johnny Brown, Pierluigi Bonello, and Erich Grotewold Growth Stage-Based Phenotypic Profiling of Plants Susanne Kjemtrup, Douglas C. Boyes, Cory Christensen, Amy J. McCaskill, Michelle Hylton, and Keith Davis Index

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