Plant functional genomics
著者
書誌事項
Plant functional genomics
(Methods in molecular biology / John M. Walker, series editor, v. 236)
Humana Press, c2003
- e-book
大学図書館所蔵 件 / 全18件
-
該当する所蔵館はありません
- すべての絞り込み条件を解除する
注記
Includes bibliographical references and index
内容説明・目次
内容説明
Functional genomics is a young discipline whose origin can be traced back to the late 1980s and early 1990s, when molecular tools became available to determine the cellular functions of genes. Today, functional genomics is p- ceived as the analysis, often large-scale, that bridges the structure and organi- tion of genomes and the assessment of gene function. The completion in 2000 of the genome sequence of Arabidopsis thaliana has created a number of new and exciting challenges in plant functional genomics. The immediate task for the plant biology community is to establish the functions of the approximately 25,000 genes present in this model plant. One major issue that will remain even after this formidable task is c- pleted is establishing to what degree our understanding of the genome of one model organism, such as the dicot Arabidopsis, provides insight into the or- nization and function of genes in other plants. The genome sequence of rice, completed in 2002 as a result of the synergistic interaction of the private and public sectors, promises to significantly enrich our knowledge of the general organization of plant genomes. However, the tools available to investigate gene function in rice are lagging behind those offered by other model plant systems. Approaches available to investigate gene function become even more limited for plants other than the model systems of Arabidopsis, rice, and maize.
目次
Part I. Finding Genes in Complex Plant Systems
An Improved Method for Plant BAC Library Construction
Meizhong Luo and Rod A. Wing
Constructing Gene-Enriched Plant Genomic Libraries Using Methylation Filtration Technology
Pablo D. Rabinowicz
RescueMu Protocols for Maize Functional Genomics
Manish N. Raizada
Precious Cells Contain Precious Information: Strategies and Pitfalls in Expression Analysis from a Few Cells
Isabelle M. Henry and Dina F. Mandoli
Combined ESTs from Plant-Microbe Interactions: Using GC Counting to Determine the Species of Origin
Edgar Huitema, Trudy A. Torto, Allison Styer, and Sophien Kamoun
Part II. In Silico Prediction of Plant Gene Function
Computer Software to Find Genes in Plant Genomic DNA
Ramana V. Davuluri and Michael Q. Zhang
Genomic Colinearity as a Tool for Plant Gene Isolation
Wusirika Ramakrishna and Jeffrey L. Bennetzen
Using Natural Allelic Diversity to Evaluate Gene Function
Sherry R. Whitt and Edward S. Buckler, IV
Quantitative Trait Locus Analysis as a Gene Discovery Tool
Michael D. McMullen
Part III. Forward and Reverse Genetic Strategies
Transposon Tagging Using Activator (Ac) in Maize
Thomas P. Brutnell and Liza J. Conrad
T-DNA Mutagenesis in Arabidopsis
Jose M. Alonso and Anna N. Stepanova
Physical and Chemical Mutagenesis
Andrea Kodym and Rownak Afza
High-Throughput TILLING for Functional Genomics
Bradley J. Till, Trenton Colbert, Rachel Tompa, Linda C. Enns, Christine A. Codomo, Jessica E. Johnson, Steven H. Reynolds, Jorja G. Henikoff, Elizabeth A. Greene, Michael N. Steine, Luca Comai, and Steven Henikoff
Gene and Enhancer Traps for Gene Discovery
Marcela Rojas-Pierce and Patricia S. Springer
High-Throughput TAIL-PCR as a Tool to Identify DNA Flanking Insertions
Tatjana Singer and Ellen Burke
Custom Knock-Outs with Hairpin RNA-Mediated Gene Silencing
Susan Varsha Wesley, Qing Liu, Anna Wielopolska, Geoff Ellacott, Neil Smith, Surinder Singh, and Chris Helliwell
Virus-Induced Gene Silencing
S. P. Dinesh-Kumar, Radhamani Anandalakshmi, Rajendra Marathe, Michael Schiff, and Yule Liu
Exploring the Potential of Plant RNase P as a Functional Genomics Tool
Dileep K. Pulukkunat, M. L. Stephen Raj, Debasis Pattanayak, Lien B. Lai, and Venkat Gopalan
Maintaining Collections of Mutants for Plant Functional Genomics
Randy Scholl, Martin M. Sachs, and Doreen Ware
Part IV. Gain of Function Approaches
Vector Construction for Gene Overexpression as a Tool to Elucidate Gene Function
Alan Lloyd
T-DNA Activation Tagging
Johan Memelink
Part V. Phenotypic Profiling as a Tool to Determine Gene Function
Expression Profiling Using cDNA Microarrays
Suling Zhao and Wesley B. Bruce
Open Architecture Expression Profiling of Plant Transcriptomes and Gene Discovery Using GeneCalling (R) Technology
Oswald R. Crasta and Otto Folkerts
Proteomics as a Functional Genomics Tool
Ulrike Mathesius, Nijat Imin, Siria H. A. Natera, and Barry G. Rolfe
Metabolite Profiling as a Functional Genomics Tool
Anusha P. Dias, Johnny Brown, Pierluigi Bonello, and Erich Grotewold
Growth Stage-Based Phenotypic Profiling of Plants
Susanne Kjemtrup, Douglas C. Boyes, Cory Christensen, Amy J. McCaskill, Michelle Hylton, and Keith Davis
Index
「Nielsen BookData」 より