Membrane transporters : methods and protocols
著者
書誌事項
Membrane transporters : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, v. 227)
Humana Press, c2003
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Studies of membrane transporters have had great impact on our und- standing human diseases and the design of effective drugs. About 30% of current clinically marketed drugs are targeting membrane transporters or channels. Membrane Transporters: Methods and Protocols provides various practical methodologies for the ongoing research on membrane transporters. To provide readers the most up-to-date information, several emerging fields and methodologies are embraced in this book, including pharmacogenomics, bioin-formatics, and microarray technology. Pharmacogenomics studies of membrane transporters are useful in drug discovery and in predicting drug responses in the clinic. In this volume, the current status of pharma- genomics studies of transporters is reviewed and research methodologies in this field are described. Transporter classification is important in studying the structure and function of membrane transporters and has thus triggered intensive interest in recent years. Membrane Transporters: Methods and Protocols provides a systematic classification of all transmembrane transport proteins found in living org- isms on Earth. This classification system will be helpful for further studies on various aspects of membrane transporters, especially for such large-scale gene expression studies as those employing microarray technologies. Bioinformatics is frequently used in transporter studies and has become indispensable for all kinds of research methods. Commonly used bio- formatics methods, such as databases and tools for sequence analysis and - tif studies, are explained in order to facilitate membrane transporters research. Because of heterogeneous sources and tremendous amounts of data, data in- gration has become one of the most important issues in transporter studies.
目次
Pharmacogenomics of Membrane Transporters: An Overview
Qing Yan
The IUBMB-Endorsed Transporter Classification System
Wolfgang Busch and Milton H. Saier, Jr.
Bioinformatics and Data Integration in Membrane Transporter Studies
Qing Yan
DNA Microarrays to Analyze Gene Expression in Animals With Altered Transporter Expression
Gary W. Miller
Microarray Data Analysis in Studies of Membrane Transporters
Donglei Hu
Laser Capture Microdissection to Examine Transporter Expression in Specific Cell Regions
Okkyung Rho and Gary W. Miller
Site-Directed Mutagenesis in the Study of Membrane Transporters
Renae M. Ryan, Ann D. Mitrovic, and Robert J. Vandenberg
Fluorescence Techniques for Studying Membrane Transport Proteins: The P-Glycoprotein Multidrug Transporter
Frances J. Sharom, Paula L. Russell, Qin Qu, and Peihua Lu
Reconstitution of Membrane Transporters
Frances J. Sharom and Paul D. W. Eckford
Equilibrium Binding and Transport Studies
Ana M. Ojeda, Dawn T. Bravo, Terry L. Hart, and Stanley M. Parsons
Electrophysiological Studies of Ion Channels and Therapeutic Potential with Adenovirus-Mediated Gene Transfer
Jihong Qu, Thai V. Pham, and Maria N. Obreztchikova
Antibody Production and Immunocytochemical Localization of Amino Acid Transporters
David V. Pow, Robert Sullivan, and Heather Scott
Xenopus laevis Oocytes
Stefan Broeer
Measurement of Intracellular pH
Frederick B. Loiselle and Joseph R. Casey
Small-Angle X-Ray Scattering Studies of Membrane Transporter Peripheral Components and Soluble Domains
Brian H. Shilton
Membrane Protein NMR Studies
Stanley J. Opella
Analysis of Soluble Sugar Permease Domains by Solution NMR
Thomas A. Wilkinson and Yuan Chen
Molecular Modeling and Molecular Dynamics Simulationsof Membrane Transporter Proteins
Donard S. Dwyer
Imaging Plasma Membrane Proteins by Confocal Microscopy
Stella W. Y. Tsang and Peter M. Haney
Index
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