Directed evolution library creation : methods and protocols
著者
書誌事項
Directed evolution library creation : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, v. 231)
Humana Press, c2003
- alk. paper
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Biological systems are very special substrates for engineering-uniquely the products of evolution, they are easily redesigned by similar approaches. A simple algorithm of iterative cycles of diversification and selection, evolution works at all scales, from single molecules to whole ecosystems. In the little more than a decade since the first reported applications of evolutionary design to enzyme engineering, directed evolution has matured to the point where it now represents the centerpiece of industrial biocatalyst development and is being practiced by thousands of academic and industrial scientists in com- nies and universities around the world. The appeal of directed evolution is easy to understand: it is conceptually straightforward, it can be practiced without any special instrumentation and, most important, it frequently yields useful solutions, many of which are totally unanticipated. Directed evolution has r- dered protein engineering readily accessible to a broad audience of scientists and engineers who wish to tailor a myriad of protein properties, including th- mal and solvent stability, enzyme selectivity, specific activity, protease s- ceptibility, allosteric control of protein function, ligand binding, transcriptional activation, and solubility. Furthermore, the range of applications has expanded to the engineering of more complex functions such as those performed by m- tiple proteins acting in concert (in biosynthetic pathways) or as part of mac- molecular complexes and biological networks.
目次
Part I. Mutagenesis and Recombination Methods
Generating Mutant Libraries Using Error-Prone PCR
Patrick C. Cirino, Kimberly M. Mayer, and Daisuke Umeno
Preparing Libraries in Escherichia coli
Alexander V. Tobias
Preparing Libraries in Saccharomyces cerevisiae
Thomas Bulter and Miguel Alcalde
Creating Random Mutagenesis Libraries by Megaprimer PCR of Whole Plasmid (MEGAWHOP)
Kentaro Miyazaki
Construction of Designed Protein Libraries Using Gene Assembly Mutagenesis
Paul H. Bessette, Marco A. Mena, Annalee W. Nguyen, and Patrick S. Daugherty
Production of Randomly Mutated Plasmid Libraries Using Mutator Strains
Annalee W. Nguyen and Patrick S. Daugherty
Evolution of Microorganisms Using Mutator Plasmids
Olga Selifonova and Volker Schellenberger
Random Insertion and Deletion Mutagenesis
Hiroshi Murakami, Takahiro Hohsaka, and Masahiko Sisido
Random Oligonucleotide Mutagenesis
Jessica L. Sneeden and Lawrence A. Loeb
Saturation Mutagenesis
Radu Georgescu, Geethani Bandara, and Lianhong Sun
DNA Shuffling
John M. Joern
Family Shuffling with Single-Stranded DNA
Wenjuan Zha, Tongbo Zhu, and Huimin Zhao
In Vitro DNA Recombination by Random Priming
Olga Esteban, Ryan D. Woodyer, and Huimin Zhao
Staggered Extension Process (StEP) In Vitro Recombination
Anna Marie Aguinaldo and Frances H. Arnold
RACHITT: Gene Family Shuffling by Random Chimeragenesis on Transient Templates
Wayne M. Coco
The Creation of ITCHY Hybrid Protein Libraries
Marc Ostermeier and Stefan Lutz
Preparation of SCRATCHY Hybrid Protein Libraries: Size- and In-Frame Selection of Nucleic Acid Sequences
Stefan Lutz and Marc Ostermeier
Sequence Homology-Independent Protein Recombination (SHIPREC)
Andrew K. Udit, Jonathan J. Silberg,and Volker Sieber
Producing Chimeric Genes by CLERY: In Vitro and In Vivo Recombination
Valerie Abecassis, Denis Pompon, and Gilles Truan
Part II. Analysis of Library Diversity
Analysis of Shuffled Libraries by Oligonucleotide Probe Hybridization
Peter Meinhold, John M. Joern, and Jonathan J. Silberg
Sequence Mapping of Combinatorial Libraries on Macro- or Microarrays: Experimental Design of DNA Arrays
Valerie Abecassis, Gilles Truan, Loic Jaffrelo, and Denis Pompon
Sequence Mapping of Combinatorial Libraries on Macro- and Microarrays: Bioinformatic Treatment of Data
Denis Pompon, Gilles Truan, and Valerie Abecassis
Index
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