Cell structure
著者
書誌事項
Cell structure
（The practical approach series, no. 262 . Essential cell biology : a practical approach ; v. 1）
Oxford University Press, 2003
- : pbk
- : hbk
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注記
Includes bibliographical references and index
内容説明・目次
- 巻冊次
-
: hbk ISBN 9780199638307
内容説明
Biological and medical research relies upon an integrated understanding of the molecules within cells and of the interactions between cells. This has imposed great demands on investigators. Being an expert in a relatively narrow area is no longer sufficient as many studies now require the use of a wide range of techniques to provide the necessary integration. A lack of familiarity with the experimental possibilities can make such diversification difficult to
achieve. This two volume set of Essential Cell Biology is designed to help researchers overcome these problems. It has not been possible to include all of the techniques available in cell biology so the challenge was to identify those that might be most relevant to researchers who are new to this topic.
We have tried to cover both traditional and more recent approaches. The theory and basic principles of each technique are described, together with detailed protocols and advice for trouble shooting. Directions to more specialised techniques are also included. We hope the result inspires readers to experience the challenges and rewards of cell biology research for themselves and to contribute to the ongoing task of understanding the life of the cell.
Essential Cell Biology volume 1 focuses on techniques for studying cell structure whilst volume 2 concentrates on understanding how the cell functions. Volume 1 details the essential background information and protocols for observing and understanding cell morphology and cell structure, including, for example, investigations of nucleic acids, lipids, and the cytoskeleton. This is the essential guide to cell biology for researchers new to the field.
目次
- 1. Cell structure
- 2. Basic light microscopy
- 3. Electron microscopy in cell biology
- 4. Subcellular Fractionations
- 5. Plant Cell Biology
- 6. Protein Purification
- 7. Gel Electrophoresis of Proteins
- 8. Biophysical methods in structural cell biology
- 9. Nucleic acids
- 10. Cell lipids: from isolation to funational dynamics
- 11. Extracellular matrix protocols for the study of complex phenotypes
- 12. The cytoskeleton
- 巻冊次
-
: pbk ISBN 9780199638314
内容説明
Recent advances in our understanding of cells has put cell biology at the center of biological and medical research. This two volume set provides researchers with the information they need to understand and carry out the essential techniques used for studying cells. It covers a wide range of traditional and recently developed techniques and includes the fine detail necessary for immediate application in the laboratory. It is useful both as a compendium of protocols
for experienced researchers and as a valuable guide for newcomers to the subject.
目次
- 1. Cell structure
- 2. Basic light microscopy
- 3. Electron microscopy in cell biology
- 4. Subcellular Fractionations
- 5. Plant Cell Biology
- 6. Protein Purification
- 7. Gel Electrophoresis of Proteins
- 8. Biophysical methods in structural cell biology
- 9. Nucleic acids
- 10. Cell lipids: from isolation to funational dynamics
- 11. Extracellular matrix protocols for the study of complex phenotypes
- 12. The cytoskeleton
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