Gene biotechnology

著者

    • Wu, William

書誌事項

Gene biotechnology

William Wu ... [et al.]

CRC Press, c2004

2nd ed

統一タイトル

Methods in gene biotechnology

大学図書館所蔵 件 / 全4件

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注記

First ed. published under title: Methods in gene biotechnology. Boca Raton : CRC Press, 1997

Includes bibliographical references and index

内容説明・目次

内容説明

Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development. Covering state-of-the-art technologies and a broad range of practical applications, the Second Edition of Gene Biotechnology presents information that researchers and students need to understand and apply today's biotechnology techniques. It discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies, including gene manipulation methods. The book contains clearly detailed, step-by-step protocols with helpful troubleshooting tips. It addresses the needs of researchers in academic and commercial environments, and serves as a guide for graduate students designing, implementing, and evaluating experimental projects. Written by a team of internationally recognized scientists, this edition offers several notable features typically lacking in other works on the subject. The book presents protocols, as well as clear and simple explanations of the key principles and concepts behind the methods. It includes useful troubleshooting guides to help solve experimental problems, presenting a single, logically organized source for all the most important new methodologies. This unique resource provides the tools for success in molecular and cellular biology research today.

目次

Approaches to Novel Research. Rapid Isolation of Specific cDNAs or Genes by PCR New! High Throughput mRNA Profiling via cDNA Microarrays or DNA Chips New! mRNA Profiling by Quantitative RT-PCR Technique New! Functional Genome vs. Drug Discovery Construction and Screening of Subtracted and Complete Expression cDNA Libraries Subcloning of Gene or cDNAs Nonisotopic and Isotopic DNA or RNA Sequencing Information Superhighway and Computer Databases of Nucleic Acids and Proteins Characterization of DNA or Genes by Southern Blot Hybridization Gene Overexpression by Sense RNA in Mammalian Systems Analysis of Gene Expression at the RNA Level Analysis of Gene Expression at the Protein Level New! Proteomics vs. Drug Discovery New! Phage Display Technology DNA or mRNA Analysis by Radioactive in situ Hybridization RT-PCR Detection of Low Abundance mRNAs Isolation and Characterization of Genes from Genomic DNA Libraries Culture of Mouse Embryonic Stem Cells as a Model Mammalian Cell Line for Gene Expression Studies New Strategies for Gene Knockout Generation of Random Chimeras for Functional Assays of Genes Rapid Generation of Mutant DNA Constructs by Site-Specific Mutagenesis Large-scale Expression and Purification of Recombinant Proteins

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