HPLC of peptides and proteins : methods and protocols
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書誌事項
HPLC of peptides and proteins : methods and protocols
(Methods in molecular biology / John M. Walker, series editor, v. 251)
Humana Press, c2004
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注記
Includes bibliographical references and index
HTTP:URL=http://www.loc.gov/catdir/toc/ecip047/2003017464.html Information=Table of contents
内容説明・目次
内容説明
The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
目次
Part I. Modes of HPLC
HPLC of Peptides and Proteins: Basic Theory and Methodology
Marie-Isabel Aguilar
Reversed-Phase High-Performance Liquid Chromatography
Marie-Isabel Aguilar
Ion-Exchange Chromatography
Peter Stanton
High-Performance Hydrophobic Interaction Chromatography
Kalman Benedek
Gel Filtration Chromatography
Peter Stanton
Hydrophilic Interaction Chromatography
Herbert Lindner and Wilfried Helliger
Immobilized Metal Ion Affinity Chromatography of Proteins
Michael Zachariou
Immunoaffinity Chromatography of Proteins
Stuart R. Gallant
Liquid Chromatography-Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins
Tun-Li Shen and Kathleen R. Noon
Part II. Preparative Methodologies
Capillary Separations
Montserrat Carrascal and Joaquin Abian
Micropreparative HPLC of Peptides and Proteins
Edouard C. Nice and Marie-Isabel Aguilar
Multidimensional HPLC Purification of Proteins
Edouard C. Nice and Marie-Isabel Aguilar
Analytical High-Performance Liquid Chromatography
Kalman Benedek
Prep/Semiprep Separations of Peptides
Denis B. Scanlon and James Finlayson
Large-Scale Protein Chromatography
Joseph Bertolini, Peter Gomme, and Patrick Thomas
Part III. Applications
Proteolytic Peptide Mapping, Peter Hojrup
Mass Spectrometric Characterization of Posttranslationally Modified Proteins-Phosphorylation
Martin R. Larsen
Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry
Nana Kawasaki, Miyako Ohta, Satsuki Itoh, and Takao Hayakawa
HPLC in the Analysis of Peptide Metabolism
Rebecca A. Lew
Isolation and Characterization of Naturally ProcessedMHC-Bound Peptides From the Surface of Antigen-Presenting Cells
Anthony W. Purcell
Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques
David Wilson and Paul Alewood
HPLC and Mass Spectrometry of Intrinsic Membrane Proteins
Julian P. Whitelegge
IgG Purification
Maree S. Powell and Bruce D. Wines
DNA-Binding Proteins: LC-MS to Identify Key Domains in RNA Polymerase-Promoter Interactions
Malcolm Buckle
Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC
Jordi Gomez-Ramirez, Isaac Blanco, and Jordi Ortiz
Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry
Jean-Louis Aubagnac, Christine Enjalbal, Jean Martinez, Pierre Sanchez, and Gilles Subra
Proteome Analysis
Matthew J. Powell and Aaron T. Timperman
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