Microscopy and histology for molecular biologists : a user's guide

著者

書誌事項

Microscopy and histology for molecular biologists : a user's guide

edited by J.A. Kiernan and I. Mason

Portland Press, c2002

大学図書館所蔵 件 / 全3件

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Research workers in biochemistry, cell biology, developmental biology, immunology, molecular biology, and neuroscience frequently need to examine cells or tissues microscopically. Unfortunately, many of them lack the appropriate education and technical training that are needed to use even the simplest microscope properly. The principles that govern the preparation of specimens also are commonly not known or badly misunderstood. This text informs research workers how to prepare cells and tissues for examination by traditional and modern types of light microscopy, and how to obtain and analyze images. The authors provide reasoned accounts of mechanisms and procedures, with selected references to original sources, review articles and textbooks. In addition, detailed technical instructions are provided for many important and frequently used methods. The theoretical background is also presented in sufficient detail to allow intelligent modification of these techniques to suit particular investigations to solve unexpected problems. Chapters are written by experts in their own fields (histotechnology or microscopy) for readers who are (or aspire to be) experts in biological or biochemical research. 2

目次

  • Part 1 Methods of light microscopy: methods of light photomicroscopy
  • digital and confocal photomicrography
  • three-dimensional microscopy
  • photomicrography. Part 2 Tissue preparation, staining and histochemistry: fluorescent staining of living cells
  • freezing and fixation
  • tissue processing and sectioning
  • staining and counterstaining to show structural features
  • immunohistochemical methods
  • staining skeletons of whole animals
  • metabolic mapping by enzyme histochemistry
  • detection of cell division and cell death
  • in situ hybridization to RNA in tissue sections
  • in situ hybridization and sectioning of whole embryos and tissues.

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