p53 protocols
Author(s)
Bibliographic Information
p53 protocols
(Methods in molecular biology / John M. Walker, series editor, v. 234)
Humana, c2003
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal the unique structural features and biochemical functions of this protein. Several unique properties of p53 posed a challenge to understa- ing its normal function in the initial phase of its research. The low levels of p53 in normal cells, its stabilization under situations of genotoxic stress, induction of growth arrest, and apoptosis with stabilization of the protein, obstructed the visibility of its normal, unmutated function. The property of p53 that can sense a promoter and transactivate or inhibit is still not well understood. It is still not known whether it is the absence of the protein that causes tumorigenesis, or if its mutants have a dominant role in inducing cancer. p53 Protocols comprises eighteen chapters for the study of the diverse properties of p53 and related proteins. The methods included are invaluable for delineating the function of other proteins that may function as tumor suppr- sors or growth suppressors. The chapters are not presented in any schematic order, for the importance and diversity of the functions of p53 make it imp- sible to organize them suitably. We have made a sincere effort to collect the methods most useful to those investigators working on tumor suppressors or growth suppressors. The purpose of p53 Protocols is not only to provide investigators with methods to analyze similar biochemical functions, but also to familiarize them with the associated problems that arose during the course of investigations.
Table of Contents
Adenovirus Expressing p53
Matthew Holmes, Elizabeth Rosenberg, and Kristoffer Valerie
Purification of Recombinant p53 from Sf9 Insect Cells
Xiu Zhu Sun, John Nguyen, and Jamil Momand
Generation and Characterization of p53 Mutant Mice
Melissa L. Dumble, Lawrence A. Donehower, and Xiongbin Lu
Identification of p53 Target Genes by Fluorescent Differential Display
Susanne Stein, Yong-Jig Cho, Roger S. Jackson II, and Peng Liang
p53-Induced Gene Expression Analysis
Joanna K. Sax and Wafik S. El-Deiry
Real-Time Polymerase Chain Reaction Quantitation of Relative Expression of Genes Modulated by p53 Using SYBR (R) Green I
Katherine E. Roth Stagliano, Evie Carchman, and Sumitra Deb
Transactivation and Transrepression Studies with p53
Mariano J. Scian, Rebecca Frum, Swati Palit Deb, and Sumitra Deb
Methods to Study p53-Repressed Promoters
Patrick Dumont, Anthony Della Pietra, and Maureen E. Murphy
Interaction of p53 with Cellular Proteins
David M. Keller, Shelya X. Zeng, and Hua Lu
Interaction of p53 with the Adenovirus E1B-55 kDa Protein
Judith Roth and Matthias Dobbelstein
In Vivo Footprinting and DNA Affinity Chromatography for Analysis of p53 DNA Binding Ability
Maria Patricia Molina, Christine Cain, and Jill Bargonetti
Signaling to p53: The Use of Phospho-Specific Antibodies to Probe for In Vivo Kinase Activation
Ashley L. Craig, Susan E. Bray, Lee E. Finlan, Neil M. Kernohan, and Ted R. Hupp
Detection of Mitochondrial Localization of p53
Motohiro Mihara and Ute M. Moll
Analysis of Nuclear and Cytoplasmic Degradation of p53 in Cells after Stress
Troy W. Joseph and Ute M. Moll
Mutational Analysis of p53 in Human Tumors: Direct DNA Sequencing and SSCP
Susan Erster, Neda Slade, and Ute M. Moll
Mutational Analysis of p53 in Human Tumors:Immunocytochemistry
Neda Slade and Ute M. Moll
Flow Cytometric Analysis of p53-Induced Apoptosis
Michael Berger and Ygal Haupt
Flow Cytometric Analysis of MDM2-Mediated Growth Arrest
Rebecca Frum and Swati Palit Deb
Index
by "Nielsen BookData"