Drosophila cytogenetics protocols
Author(s)
Bibliographic Information
Drosophila cytogenetics protocols
(Methods in molecular biology / John M. Walker, series editor, v. 247)
Humana, c2004
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.
Table of Contents
The Chromosomes of Drosophila melanogaster
Daryl S. Henderson
Spermatogenesis: Analysis of Meiosis and Morphogenesis
Helen White-Cooper
Time-Lapse Imaging of Male Meiosis by Phase-Contrast and Fluorescence Microscopy
Elena Rebollo and Cayetano Gonzalez
Immunocytological Analysis of Oogenesis
Endre Mathe
Cytological Analysis of Oogenesis
Seppo Nokkala and Christina Nokkala
Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants
Dmitry E. Koryakov, Natalia I. Mal'ceva, Robert C. King, and Igor F. Zhimulev
Feulgen-DNA Cytophotometry for Estimating C Values
Ellen M. Rasch
Fluorescent BrdU Labeling and Nuclear Flow Sorting of the Drosophila Ovary
Brian R. Calvi and Mary A. Lilly
Studying Nuclear Organization in Embryos Using Antibody Tools
Kristen M. Johansen and Jorgen Johansen
Whole-Mount Fluorescence In Situ Hybridization to Chromosomes of Embryos
Daryl S. Henderson
Orcein Staining and the Identification of Polytene Chromosomes
John Tonzetich
Salivary Chromosome Analysis of Aberrations
Adelaide T. C. Carpenter
In Situ Hybridization to Polytene Chromosomes
Robert D. C. Saunders
Combined Immunostaining and FISH Analysis of Polytene Chromosomes
Sergey Lavrov, Jerome Dejardin, and Giacomo Cavalli
Electron Microscopy of Polytene Chromosomes
Valery F. Semeshin, Elena S. Belyaeva, Victor V. Shloma, and Igor F. Zhimulev
Analysis of Mitosis in Squash Preparations of Larval Brains: Orcein, Giemsa, Hoechst 33258, DAPI, Quinacrine, and N-Banding
Laura Fanti and Sergio Pimpinelli
Analyzing Chromosome Function by High Frequency Formation of Dicentric Chromosomes In Vivo
Kami Ahmad and Kent G. Golic
Analysis of Chromosomes of the Larval CNS by FISH and BrdU Labeling
Amy K.Csink
Immunostaining of Squash Preparations of Chromosomes of Larval Brains
Laura Fanti and Sergio Pimpinelli
Visualizing Mitosis in Whole-Mount Larval Brains
Daryl S. Henderson
Immunostaining of Whole-Mount Imaginal Discs
Brigitte de Saint Phalle
Wing Somatic Mutation and Recombination Test
Heloisa Helena Rodrigues de Andrade, Maria Luiza Reguly, and Mauricio Lehmann
Analysis of Histoblasts
Mekkara Mandaravally Madhavan and Kornath Madhavan
Visualizing Apoptosis
Kimberly McCall, Jason S. Baum, Kristen Cullen, and Jeanne S. Peterson
RNAi in Cultured Drosophila Cells
Ling-Rong Kao and Timothy L. Megraw
Index
by "Nielsen BookData"