Nonviral gene transfer techniques
著者
書誌事項
Nonviral gene transfer techniques
(Methods in molecular biology / John M. Walker, series editor, v. 245 . Gene delivery to mammalian cells ; v. 1)
Humana, c2004
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.
目次
Part I. Delivery Using Chemical Methods
Chemical Methods for DNA Delivery: An Overview
Dexi Liu, Evelyn F. Chiao, and Hui Tian
Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran
Gregory S. Pari and Yiyang Xu
DNA Delivery to Cells in Culture Using Peptides
Lei Zhang, Nicholas Ambulos, and A. James Mixson
DNA Delivery to Cells in Culture Using PNA Clamps
Todd D. Giorgio and Shelby K. Wyatt
Dendrimer-Mediated Cell Transfection In Vitro
James R. Baker, Jr., Anna U. Bielinska, and Jolanta F. Kukowska-Latallo
DNA Delivery to Cells in Culture Using Cationic Liposomes
Shelby K. Wyatt and Todd D. Giorgio
Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium
John Marshall, Nelson S. Yew, and Seng H. Cheng
Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium
Young K. Song, Guisheng Zhang, and Dexi Liu
Delivery of DNA to Tumor Cells Using Cationic Liposomes
Duen-Hwa Yan, Bill Spohn, and Mien-Chie Hung
Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes
Lalitha R. Belur and R. Scott McIvor
Part II. Delivery Using Physical Methods
Gene Delivery Using Physical Methods: An Overview
Te-hui W. Chou, Subhabrata Biswas, and Shan Lu
Gene Delivery to Mammalian Cells by Microinjection
Robert King
Delivery of DNA to Cells in Culture Using Particle Bombardment
William C. Heiser
Delivery of DNA to Skin by Particle Bombardment
Shixia Wang, Swati Joshi, and Shan Lu
Biolistic Transfection of Cultured Organotypic Brain Slices
A. Kimberley McAllister
Efficient Electroporation of Mammalian Cells in Culture
Peter A. Barry
Delivery of DNA to Skin by Electroporation
Nathalie Dujardin and Veronique Preat
In VivoDNA Electrotransfer in Skeletal Muscle
Guenhael Sanz, Saulius Satkauskas, and Lluis M. Mir
Electrically Mediated Plasmid DNA Delivery to Solid Tumors In Vivo
Mark J. Jaroszeski, Loree C. Heller, Richard Gilbert, and Richard Heller
Hydrodynamic Delivery of DNA
Joseph E. Knapp and Dexi Liu
Naked DNA Gene Transfer in Mammalian Cells
Guofeng Zhang, Vladimir G. Budker, James J. Ludtke, and Jon A. Wolff
Microparticle Delivery of Plasmid DNA to Mammalian Cells
Mary Lynne Hedley and Shikha P. Barman
DNA Delivery to Cells in Culture Using Ultrasound
Thomas P. McCreery, Robert H. Sweitzer, and Evan C. Unger
DNA Delivery to Cells In Vivo by Ultrasound
Thomas P. McCreery, Robert H. Sweitzer, Evan C. Unger, and Sean Sullivan
Index
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