Mobile genetic elements : protocols and genomic applications
著者
書誌事項
Mobile genetic elements : protocols and genomic applications
（Methods in molecular biology / John M. Walker, series editor, v. 260）
Humana Press, c2004
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Mobile Genetic Elements: Protocols and Genomic Applications brings together a wide array of transposon-based protocols and stategies for studying genome structure, function, and evolution into a highly practical, single-source volume. Such transposable element (TE)-derived techniques have been applied succe- fully for a variety of purposes ranging from mutagenesis, gene silencing, transgenesis, and their use as polymorphic marker systems. To our knowledge no such synthesis has been presented before. Chapters 2-4 provide a series of DNA hybridization techniques for anal- ing the distribution and dynamics of mobile DNAs at the hosts' genomic level. With the current revolution in genomics and the availability of complete genome sequences, computational analyses provide an extremely powerful tool for i- lating and investigating TEs at the in silico level (Chapter 5). For the analyses of transpositional mechanisms at the biochemical level Chapter 6 provides a detailed protocol for LTR retrotransposons in heterologous host systems. Ch- ters 7-10 are focused on TE-based mutagenesis protocols for studying gene functions in a broad range of organisms.
Based on their ubiquitous nature and their activity in creating genomic diversity by integrating novel DNA segments into genomes, TEs provide highly informative sets of polymorphic markers (Chapters 11-13). Finally, the last two chapters are dedicated to their technical applications during transgenesis in arthropods and vertebrates.
目次
Mobile Genetic Elements as Natural Tools for Genome Evolution
Wolfgang J. Miller and Pierre Capy
Detection of Transposable Elements in Drosophila Salivary Gland Polytene Chromosomes by In Situ Hybridization
Christian Biemont, Laurence Monti-Dedieu, and Francoise Lemeunier
Fluorescent In Situ Hybridization With Transposable Element Probes to Mitotic Chromosomal Heterochromatin of Drosophila
Patrizio Dimitri
Southern Blot Analysis of Individual Drosophila Flies
Nikolaj Junakovic
Computational Analysis of Transposable Element Sequences
I. King Jordan and Nathan J. Bowen
Biochemical Analysis of Long Terminal Repeat Retrotransposons
Andreas Bachmair, Karin Garber, Shin Takeda, Kazuhiko Sugimoto, Tetsuji Kakutani, and Hirohiko Hirochika
Tn5 as a Molecular Genetics Tool: In Vitro Transposition and the Coupling of In Vitro Technologies With In Vivo Transposition
William S. Reznikoff, Igor Y. Goryshin, and Jerry J. Jendrisak
Gene Targeting in Drosophila
Gregory B. Gloor
TE-Based Mutagenesis Systems in Plants: A Gene Family Approach
Michiel Vandenbussche and Tom Gerats
The Use of Double-Stranded RNA to Knock Down Specific Gene Activity
Mary K. Montgomery
The Application of LTR Retrotransposons as Molecular Markers in Plants
Alan H. Schulman, Andrew J. Flavell, and T. H. Noel Ellis
MITE Display
Alexandra M. Casa, Alexander Nagel, and Susan R. Wessler
Retroposon Mapping in Molecular Systematics
Norihiro Okada, Andrew M. Shedlock, and Masato Nikaido
Transformation Systems in Insects
David A. OBrochta and Peter W. Atkinson
Transposable Elements for Transgenesis and Insertional Mutagenesis in Vertebrates: A Contemporary Review of Experimental Strategies
Zoltan Ivics and Zsuzsanna Izsvak
Index
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