Superantigen protocols

著者

    • Krakauer, Teresa

書誌事項

Superantigen protocols

edited by Teresa Krakauer

（Methods in molecular biology / John M. Walker, series editor, v. 214）

Humana Press, c2003

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Superantigen Protocols assembles experimental protocols that have proved useful for the study of superantigens. These techniques will allow researchers from various areas of cell biology, microbiology, immunology, biochemistry, and molecular biology to assess the physical characteristics and biological effects of well-known superantigens as well as of putative substances that might have superantigenic activities, and to explore therapies for superantig- induced effects. Microbial exotoxins have been studied for decades as virulence factors because of their pathogenic effects. The term "superantigen" was coined by Marrack and Kappler a decade ago for some of these molecules because of their potent T-cell stimulatory activities. In recent years, advances in mole- lar biology provide recombinant as well as natural superantigens in highly purified form for physical characterization. Superantigens are now used extensively as tools to study interactions between receptors on cells of the immune system as they bind to major histocompatibility complex class II m- ecules on antigen-presenting cells and V regions of T-cell receptors. The b- ? logical effects that result from these interactions are studied both in vitro and in vivo. The intent of this book is, therefore, to bring together up-to-date te- niques developed by experts in the field of biochemistry, immunology, and molecular biology for the study of superantigens. Superantigen Protocols begins with an overview of the field to provide background information on the various classes of superantigens and their str- ture.

目次

Superantigens: Structure, Function, and Diversity Matthew D. Baker and K. Ravi Acharya Expression, Purification, and Detection of N ovel Streptococcal Superantigens John K. McCormick and Patrick M. Schlievert Flow Cytometric Detection of MMTV Superantigens Gary Winslow Spectrophotometric Methods for the Determination of Superantigen Structure and Stability Anders Cavallin, Karin Petersson, and Goeran Forsberg Binding Kinetics of Superantigen with TCR and MHC Class II S. Munir Alam and Nicholas R. J. Gascoigne Directed Evolution of T-Cell Receptors for Binding Superantigens Hywyn R. O. Churchill and David M. Kranz Analysis of Superantigen Binding to Soluble T-Cell Receptors Hywyn R. O. Churchill and David M. Kranz Role of Accessory Molecules in the Superantigen-Induced Activation of Peripheral Blood T Cells Catherine Gelin, Marie-Therese Zilber, and Dominique Charron T-Lymphocyte Activation Induced by Staphylococcal Enterotoxin Superantigens: Analysis of Protein Tyrosine Phosphorylation Anne Roumier, Florence Niedergang, and Andres Alcover Measurement of Proinflammatory Cytokines and T-Cell Proliferative Response in Superantigen-Activated Human Peripheral Blood Mononuclear Cells Teresa Krakauer RNase Protection Assay for the Study of the Differential Effects of Therapeutic Agents in Suppressing Staphylococcal Enterotoxin B-Induced Cytokines in Human Peripheral Blood Mononuclear Cells Teresa Krakauer, Xin Chen, O. M. Zack Howard, and Howard A. Young Staining Protocol for Superantigen-Induced Cytokine Production Studied at the Single-Cell Level Lars Bjoerk Assessment of Specific T-Cell Activation by Superantigens Natalie Sutkowski and Brigitte T. Huber Superantigen-Induced Changes in Epithelial Ion Transport and Barrier Function: Use of an In VitroModel James L. Watson and Derek M. McKay Pyrogenic, Lethal, and Emetic Properties of Superantigens in Rabbits and Primates John K. McCormick, Gregory A. Bohach, and Patrick M. Schlievert Index

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