MHC protocols

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Bibliographic Information

MHC protocols

edited by Stephen H. Powis, Robert W. Vaughan

（Methods in molecular biology / John M. Walker, series editor, v. 210）

Humana Press, c2003

Available at  / 5 libraries

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Includes bibliographical references and index

Description and Table of Contents

Description

The aim of MHC Protocols is to document protocols that can be used for the analysis of genetic variation within the human major histocompatibility complex (MHC; HLA region). The human MHC encompasses approximately 4 million base pairs on the short arm of chromosome 6 at cytogenetic location 6p21. 3. The region is divided into three subregions. The telomeric class I region contains the genes that encode the HLA class I molecules HLA-A, -B, and -C. The centromeric class II region contains the genes encoding the HLA class II molecules HLA-DR, -DQ, and -DP. In between is the class III region, originally identified because it contains genes encoding components of the complement pathway. The entire human MHC has recently been sequenced (1) and each subregion is now known to contain many other genes, a number of which have immunological functions. The study of polymorphism within the MHC is well established, because the region contains the highly polymorphic HLA genes. HLA polymorphism has been used extensively in solid organ and bone marrow transplantation to match donors and recipients. As a result, large numbers of HLA alleles have been identified, a process that has been further driven by recent interest in HLA gene diversity in ethnic populations. The extreme genetic variation in HLA genes is believed to have been driven by the evolutionary response to infectious agents, but relatively few studies have analyzed associations between HLA genetic variation and infectious disease, which has been difficult to demonstrate.

Table of Contents

I. Databases HLA Informatics: Accessing HLA Sequences from Sequence Databases James Robinson and Steven G. E. Marsh Accessing HLA Sequencing Data Through the 6ace Database Roger Horton and Stephan Beck II. Polymorphism in Classical and Nonclassical HLA Genes HLA Typing by Restriction Fragment Length Polymorphism Analysis Robert W. Vaughan PCR-Restriction Fragment Length Polymorphism Typing of Class I and II Alleles Robert W. Vaughan PCR-Sequence-Specific Oliogonucleotide Probe Typing for HLA-A, -B, and -DR Derek Middleton and F. Williams HLA-DPA1 and -DPB1 Typing Using the PCR and Nonradioactive Sequence-Specific Oligonucleotide Probes Lori L. Steiner, Priscilla V. Moonsamy, Teodorica L. Bugawan, and Ann B. Begovich PCR-Sequence-Specific Primer Typing of HLA Class I and Class II Alleles Mike Bunce HLA Typing With Reference Strand-Mediated Conformation Analysis J. Rafael Arguello, Martha Perez-Rodriguez, Andrea Pay, Gaby Fisher, Alasdair McWhinnie, and J. Alejandro Madrigal Sequencing Protocols for Detection of HLA Class I Polymorphism Paul P. J. Dunn, Steven T. Cox, and Ann-Margaret Little HLA-E and HLA-G Typing Jorge Martinez-Laso, Eduardo Gomez-Casado, and Antonio Arnaiz-Villena Typing Alleles of HLA-DM Helene Teisserenc III. Polymorphism in Non-HLA MHC Genes Typing Alleles of TAP1 and TAP2 Stephen H. Powis Determining Alleles of the C2 Gene by Southern Blotting Zeng-Bian Zhu and John E. Volanakis Complement C4 Protein and DNA Typing Methods Peter M. Schneider and Gottfried Mauff Typing of Tumor Necrosis Factor Alleles Anthony Gerard Wilson Molecular Typing of the MHC Class I Chain-Related Gene Locus R. W. M. Collins, Henry A. F. Stephens, and Robert W. Vaughan IV.Microsatellites HLA Microsatellite Analysis Mary Carrington Index

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