Cellular and molecular tools
著者
書誌事項
Cellular and molecular tools
(Methods in molecular medicine / John M. Walker, series editor, 100 . Cartilage and osteoarthritis : 1)
Humana Press, c2004
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Osteoarthritis (OA), the most common form of arthritis, is generally characterized by a slowly progressive degeneration of articular cartilage, particularly in the weight-bearing joints. It has a stronger prevalence in women, and its incidence increases with age. OA is a major and growing health concern in developed countries, owing to steadily increasing life expectancy and the demand for better quality of life. Because of its chronic nature and nonfatal outcome, OA affects the growing population of the elderly over an increasing time span. Moreover, despite its relatively benign character, OA is one of the most disabling diseases; it is responsible for increasing financial and social burdens in terms of medical treatments, forced inactivity, loss of mobility, and dependence. Despite a growing awareness of OA as a medical problem that has yet to reach its maximum impact on society, there is a surprising absence of effective medical treatments beyond pain control and surgery. So far, only symptom-modifying drugs are available, while there remains a major demand for disease-modifying treatments of proven clinical efficacy. This demand will hopefully be met in the future by some of the drugs that have been pressed into development and are now at different stages of clinical investigation. Nevertheless, the current lack of effective treatments reflects a still insufficient knowledge of cartilage with respect to its metabolism, interactions with other joint tissues, and causes and mechanisms (possibly of very different nature) leading to failure of its turnover.
目次
Culture and Phenotyping of Chondrocytes in Primary Culture
Sylvie Thirion and Francis Berenbaum
Culture of Chondrocytes in Alginate Beads
Frederic De Ceuninck, Christophe Lesur, Philippe Pastoureau, Audrey Caliez, and Massimo Sabatini
Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines
Mary B. Goldring
Culture of Immortalized Chondrocytes and Their Use As Models of Chondrocyte Function
Mary B. Goldring
Generation of Pluripotent Stem Cells and Their Differentiation to the Chondrocytic Phenotype
Luis A. Solchaga, Jean F. Welter, Donald P. Lennon, and Arnold I. Caplan
Semiquantitative Analysis of Gene Expression in Cultured Chondrocytes by RT-PCR
Gaelle Rolland-Valognes
Quantification of mRNA Expression Levels in Articular Chondrocytes With PCR Technologies
Audrey McAlinden, Jochen Haag, Brigitte Bau, Pia M. Gebhard, and Thomas Aigner
RNA Extraction From Cartilage
Frederic Mallein-Gerin and Jerome Gouttenoire
Gene Expression Analysis in Cartilage by In Situ Hybridization
Frederic Mallein-Gerin and Jerome Gouttenoire
Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chondrocytes by Microarray Analysis
Thomas Aigner, Joachim Saas, Alexander Zien, Ralf Zimmer, Pia M. Gebhard, and Thomas Knorr
High-Efficiency Nonviral Transfection of Primary Chondrocytes
Jean F. Welter, Luis A. Solchaga, and Matthew C. Stewart
In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors
Jean-Noel Gouze, Martin J. Stoddart, Elvire Gouze, Glyn D. Palmer, Steven C. Ghivizzani, Alan J. Grodzinsky, and Christopher H. Evans
Changes of Chondrocyte Metabolism In Vitro: An Approach by Proteomic Analysis
Anne-Marie Freyria and Michel Becchi
Analysis of ChondrocyteFunctional Markers and Pericellular Matrix Components by Flow Cytometry
Gust Verbruggen, Jun Wang, Lai Wang, Dirk Elewaut, and Eric M. Veys
A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes
Frederic De Ceuninck and Audrey Caliez
Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants
Christophe Lesur and Massimo Sabatini
Production of Antibodies Against Degradative Neoepitopes in Aggrecan
John S. Mort and Peter J. Roughley
Immunoassays for Collagens in Chondrocyte and Cartilage Explant Cultures
R. Clark Billinghurst, Fackson Mwale, Anthony Hollander, Mirela Ionescu, and A. Robin Poole
Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes
Darryl D. D'Lima, Klaus Kuhn, and Martin K. Lotz
Expression, Activity, and Regulation of MAP Kinases in Cultured Chondrocytes
Jang-Soo Chun
Mechanical Loading of Chondrocytes Embedded in 3D Constructs: In Vitro Methods for Assessment of Morphological and Metabolic Response to Compressive Strain
David A. Lee and Martin M. Knight
In Vitro Physical Stimulation of Tissue-Engineered and Native Cartilage
Kelvin W. Li, Travis J. Klein, Kanika Chawla, Gayle E. Nugent, Won C. Bae, and Robert L. Sah
Index
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