Structure and in vivo analysis

著者

    • Frèdèric De Ceuninck
    • Massimo Sabatini
    • Philippe Pastoureau

書誌事項

Structure and in vivo analysis

edited by Frèdèric De Ceuninck, Massimo Sabatini and Philippe Pastoureau

（Methods in molecular medicine / John M. Walker, series editor, 101 . Cartilage and osteoarthritis : 2）

Humana Press, c2004

  • : softcover

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注記

Includes bibliographical references and index

"Softcover reprint of the hardcover 1st edition 2004"--T.p. verso of softcover

内容説明・目次

内容説明

An indispensable collection of updated classical and emerging techniques that promise to add critical knowledge to our understanding of cartilage metabolism in health and disease. Volume 1: Cellular and Molecular Tools describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells. Volume 2: Structure and In Vivo Analysis, offers cutting-edge procedures for the study-at the tissue level-of turnover, structure, and functioning of normal and diseased articular cartilage by invasive and noninvasive means. Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists with readily reproducible protocols for new experiments to understand-from the cellular to the animal level-the pathophysiology of cartilage and to discover molecular targets for pharmacological intervention.

目次

Generation and Use of Transgenic Mice As Models of Osteoarthritis Anna-Marja Saamanen and Eero Vuorio Development and Clinical Application in Arthritis of a New Immunoassay for Serum Type IIA Procollagen NH2 Propeptide Jean-Charles Rousseau, Linda J. Sandell, Pierre D. Delmas, and Patrick Garnero Histological and Immunohistological Studies on Cartilage James Melrose, Susan Smith, and Peter Ghosh Histochemical Visualization of the Cartilage Hyaladherins Using a Biotinylated Hyaluronan Oligosaccharide Bioaffinity Probe James Melrose and Susan Smith Methods for Cartilage and Subchondral Bone Histomorphometry Philippe Pastoureau and Agnes Chomel Laser-Mediated Microdissection As a Tool for Molecular Analysis in Arthritis Martin Judex, Elena Neumann, Steffen Gay, and Ulf Muller-Ladner Analysis of Protein Distribution in Cartilage Using Immunofluorescence and Laser Confocal Scanning Microscopy Stephan Soeder, Alexander Kuhlmann, and Thomas Aigner Molecular and Biochemical Assays of Cartilage Components Caroline D. Hoemann Mechanical Characterization of Native and Tissue-Engineered Cartilage Albert C. Chen, Stephen M. Klisch, Won C. Bae, Michele M. Temple, Kevin B. McGowan, Kenneth R. Gratz, Barbara L. Schumacher, and Robert L. Sah Noninvasive Study of Human Cartilage Structure by MRI Felix Eckstein High-Resolution MRI Techniques for Studies in Small-Animal Models Olivier Beuf High-Resolution Imaging of Osteoarthritis Using Microcomputed Tomography Lydia Wachsmuth and Klaus Engelke High-Resolution Ultrasonography for Analysis of Age- and Disease-Related Cartilage Changes Amena Saied and Pascal Laugier Evaluation of Cartilage Composition and Degradation by High-Resolution Magic-Angle Spinning Nuclear Magnetic Resonance Jurgen Schiller, Daniel Huster, BeateFuchs, Lama Naji, Joern Kaufmann, and Klaus Arnold Pulsed-Field Gradient-Nuclear Magnetic Resonance (PFG NMR) to Measure the Diffusion of Ions and Polymers in Cartilage: Applications in Joint Diseases Jurgen Schiller, Lama Naji, Robert Trampel, Wilfred Ngwa, Robert Knauss, and Klaus Arnold Dynamics of Collagen in Articular Cartilage Studied by Solid-State NMR Methods Daniel Huster, Jurgen Schiller, and Klaus Arnold Computerized Protocol for Anatomical and Functional Studies of Joints Sandra Martelli and Stefano Zaffagnini Index

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