B cell protocols
著者
書誌事項
B cell protocols
（Methods in molecular biology / John M. Walker, series editor, v. 271）
Humana Press, c2004
大学図書館所蔵 全11件
注記
Includes bibliographical references and index
内容説明・目次
内容説明
B-lymphocyte development and function remains an exciting area of research for those interested in the physiology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side worker to identify a reliable experimental protocol in the ocean of the literature. The aim of B Cell Protocols is to provide a collection of diverse protocols ranging from the latest inventions and applications to some classic, but still frequently used methods in B-cell biology. The authors of the various chapters are all highly qualified scientists who are either the inventors or expert users of these methods. Their extensive experience in mastering a particular method provides not only the step-by-step details of a reproducible protocol, but also useful troubleshooting tips that readers will appreciate in their daily work. We hope that this book will be helpful for both beginning and experienced researchers in the field in designing or modifying an experimental approach, and exploring a biological question from multiple angles.
目次
Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen
Richard R. Hardy and Susan A. Shinton
Characterization of B-Cell Maturation in the Peripheral Immune System
Rita Carsetti
Identification of B-Cell Subsets: An Exposition of 11-Color (Hi-D) FACS Methods
James W. Tung, David R. Parks, Wayne A. Moore, Leonard A. Herzenberg, and Leonore A. Herzenberg
Analysis of B-Cell Life-Span and Homeostasis
Irmgard Foerster
Differentiation of B Lymphocytes From Hematopoietic Stem Cells
Paulo Vieira and Ana Cumano
Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System
Faith M. Young, Carl A. Pinkert, and Andrea Bottaro
Conditional Gene Mutagenesis in B-Lineage Cells
Stefano Casola
Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells
Kai-Michael Toellner, Mahmood Khan, and Daniel Man-Yuen Sze
T-Cell-Dependent Immune Responses, Germinal Center Development, and the Anaysis of V-Gene Sequences
Claudia Berek
Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors
Kuo-I Lin and Kathryn Calame
Immunoglobulin Class Switching: In Vitro Induction and Analysis
Sven Kracker and Andreas Radbruch
In Vitro and In Vivo Assays of B-Lymphocyte Migration
Chantal Moratz and John H. Kehrl
Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo
Louise J. McHeyzer-Williams and Michael G. McHeyzer-Williams
B-Cell Signal Transduction: Tyrosine Phosphorylation, Kinase Activity, and Calcium Mobilization
Tilman Brummer, Winfried Elis, Michael Reth, and Michael Huber
Isolation of Lipid Rafts From B Lymphocytes
Anu Cherukuri, Shiang-Jong Tzeng, Arun Gidwani, Hae Won Sohn, Pavel Tolar, Michelle D. Snyder, and Susan K. Pierce
Molecular Single-Cell PCRAnalysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells
Ralf Kuppers
Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice
Helen Ferry and Richard J. Cornall
Analysis of B-Cell Signaling Using DT40 B-Cell Line
Tomoharu Yasuda and Tadashi Yamamoto
B-Cell Development and Pre-B-1 Cell Plasticity In Vitro
Antonius G. Rolink
Index
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