PCR technology : current innovations
著者
書誌事項
PCR technology : current innovations
CRC Press, c2004
2nd ed
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
A technique used to amplify the number of copies of a specific region of DNA, the polymerase chain reaction (PCR) is at the forefront of the dramatic development of biochemistry. This text provides the tools for developing innovative approaches to using this leading technology. It includes theoretical considerations, discussions, and a selection of state-of-the-art techniques for mutation studies, clinical diagnosis, and the detection of food-borne pathogens. This edition also discusses the preparation of PCR experiments, includes examples of analytical PCR divided into qualitative and quantitative applications, and explores preparative methods that address DNA generation for further analysis and in vitro evolution.
目次
SECTION I. GENERAL CONSIDERATIONSTEMPLATE ISOLATION AND PREPARATIONExtraction and Amplification of Ancient Data, Anne C. StoneLong PCR from Damaged DNA, Bernard Fromenty, PhilippeSchmitt, Dominique PessayreQuantitative mRNA Analysis in Small Cell Samples by RT-PCRand Flow Cytometry, Veronika Stemme, Sten StemmeMicrodissection of Single or Small Numbers of Cells for Analysesof DNA and RNA by PCR, Rose G. Mage, Robert F. Bonner, Harold ObiakorPCR OPTIMIZATIONPre-PCR Processing Strategies, Peter R?dstr/m, MariaL/venklev, Petra Wolffs, Charlotta L/fstr/m and RickardKnutssonNovel PCR-Enhancing Compounds and their Modes of Action, RayChakrabartiOptimizing PCR with the Aid of Experimental Design, ThomasWeissensteinerLABELINGEconomic Fluorescent Labeling of PCR-Products forMicrosatellite- and Single-Stranded-Conformation-Polymorphism (SSCP) Analysis, Markus SchuelkeCLONINGUniversal Restriction Site-Free Cloning Method Using Chimeric Primers,Guo Jun ChenSECTION II. ANALYTICAL APPLICATIONSDETECTION OF NUCLEIC ACID VARIATIONHigh-Throughput Genotyping and the PCR, Kalim U. Mir, JiannisRagoussisHigh Throughput Genetic Analysis Through Multiplexed PCR and Multi-Capillary Electrophoresis, John M Butler, Peter M. ValloneThe Good Assay: A Purification Free Assay for Genotyping by Maldi MassSpectrometry, J/rg Tost, Ram n Kucharzak, Doris Lechner, Ivo Glynne GutPrimer Design for Large Scale Multiplex PCR and Arrayed PrimerExtension (APEX), Maido Remm, Ants Kurg, Andres MetspaluSurface Plasmon Resonance Based Biosensor Technology for Real-Time Detection of PCR Products, Giordana Feriotto, Roberto GambariFluorescent Amplified Fragment Length Polymorphism (FAFLP)Genotyping - Catherine ArnoldGlobal Analysis of DNA Allelic Variations (GADAV) by Specific Enrichmentof Mismatches and Selective Amplification of Heterohybrids, XinghuaPan, Sherman M. WeissmanQuantitative DNA-Methylation Analysis by the Bisulfite ConversionMethod, Osman El-Maarri, Manfred Kuepper, Johannes Oldenburg, J/rnWalterSEQUENCINGPyrosequencing Technology, P?l Nyr?n, Tommy Nordstr/m, BabackGharizadehOctamer-Primed Cycle Sequencing, Anelia I. Kraltcheva, Susan H. HardinIN SITU ANALYSISIn Situ Amplification of cDNA, Gerard J. NuovoDetection of a Single Copy Sequence on Human by Cyclic Prins, KirstenMennicke, Sven Opitz, Eberhard SchwingerQUANTITATIVE ANALYSISMeaningful Quantification of mRNA Using Real-Time PCR, Stephen ABustinAbsolute Quantification of Specific Nucleic Acids by (RT)-PCR Using aNonlinear Mathematical Model for Data Analysis, Huong L.Vu, SergeTroubetzkoy, Vu Q. Nguyen, Michael W. Russell, Jiri MesteckySingle-Molecule PCR - Basic Protocols and Applications, Paul H. DearGene Expression and Discovery: Application of a Genome-WideApproach, Timothy A. Lohret, Karen M. KellyThe Detection of Differential Gene Expression Changes Using SAG
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