Ribozymes and siRNA protocols
著者
書誌事項
Ribozymes and siRNA protocols
(Methods in molecular biology / John M. Walker, series editor, v. 252)
Humana Press, c2004
2nd ed.
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
During the last few years, new discoveries in the RNA field have opened up a wealth of opportunities to specifically target mRNA for functional analysis and target validation. Contained in this volume of Methods in Molecular Biology are methods useful for the design and application of ribozymes, DNAzymes, and small interfering RNAs (siRNAs). In addition, a number of illustrations aiming to facilitate the und- standing and application of each method is included. Like any new field, ribozymes and siRNA research is rapidly evolving, and so too are the methods used to study, select, express, and control their structure and in vivo biological activities. Ribozymes and siRNA Protocols, Second Edition focuses on the latest technical advances in interfering with gene expression. It contains a short int- duction followed by general and specific protocols for using hammerhead ribozymes and derivates, DNAzymes, hairpin ribozymes, group I intron ribozymes, RNase P ribozymes, and siRNAs. Also in this volume are general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy protocols using ribozymes.
目次
Ribozyme- and siRNA-Mediated mRNA Degradation: A General Introduction
Mouldy Sioud
Combination of Chemical and Enzymatic RNA Synthesis
Rajesh K. Gaur, Andreas Hanne, and Guido Krupp
Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes
Martha J. Fedor
Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection
Kumud K. Singh, Andreas Hanne, and Guido Krupp
Quantification of Ribozyme Target RNA Using Real-Time PCR
Dagmar Klein, Camillo Ricordi, and Ricardo L. Pastori
Analysis of Ribozyme Structure and Function by Nucleotide Analog Interference Mapping
Soumitra Basu, Catherine Pazsint, and Gopeswar Chowdhury
Analysis of Global Conformational Transitions in Ribozymes
David M. J. Lilley
In Vivo Detection of Ribozyme Cleavage Products and RNA Structure by Use of Terminal Transferase-Dependent PCR
Hsiu-Hua Chen, Daniela Castanotto, Jeanne LeBon, John J. Rossi, and Arthur D. Riggs
Identification of Efficient Cleavage Sites in Long-Target RNAs
Wei-Hua Pan and Gary A. Clawson
Selection In Vitro of Allosteric Ribozymes
Adam Roth and Ronald R. Breaker
Regulation of Ribozyme Cleavage Activity by Oligonucleotides
Yasuo Komatsu and Eiko Ohtsuka
Tetracycline-Regulated Expression of Hammerhead Ribozymes In Vivo
Emma T. Bowden and Anna T. Riegel
Ribozyme Expression Systems
Masayuki Sano and Kazunari Taira
Design and Expression of Chimeric U1/Ribozyme Transgenes
Roger Abounader, Robert Montgomery, Harry Dietz, and John Laterra
Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases
Jason J. Fritz, Marina Gorbatyuk, Alfred S. Lewin, and William W. Hauswirth
Helicase-Attached Novel Hybrid Ribozymes
Hiroaki Kawasaki, Masaki Warashina, Tomoko Kuwabara, and Kazunari Taira
Functional Gene Discovery Using Hybrid Ribozyme Libraries
Yoshio Kato, Masaru Tsunemi, Makoto Miyagishi, Hiroaki Kawasaki, and Kazunari Taira
Maxizyme Technology
Mayu Iyo, Hiroaki Kawasaki, and Kazunari Taira
Target-Site Selection for the 10-23 DNAzyme
Murray J. Cairns and Lun-Quan Sun
In Vitro Selected RNA-Cleaving DNA Enzymes From Combinatorial Libraries
Samitabh Chakraborti, Bandi Sriram and Akhil C. Banerjea
Nucleic Acid Sequence Analysis Using DNAzymes
Murray J. Cairns and Lun-Quan Sun
Crystallization of the Hairpin Ribozyme: Illustrative Protocols
Peter B. Rupert and Adrian R. Ferre-D'Amare
An Experimental Method for Selecting Effective Target Sites and Designing Hairpin Ribozymes
Alicia Barroso-delJesus, Elena Puerta-Fernandez, Cristina Romero-Lopez, and Alfredo Berzal-Herranz
Design and Optimization of Sequence-Specific Hairpin Ribozymes
Cristina Romero-Lopez, Alicia Barroso-delJesus, Elena Puerta-Fernandez, and Alfredo Berzal-Herranz
Design, Targeting, and Initial Screening of sTRSV-Derived Hairpin Ribozymes for Optimum Helix 1 Length and Catalytic Efficiency In Vitro
Max W. Richardson, Linda Hostalek, Michelle Dobson, Jason Hu, Richard Shippy, Andrew Siwkowski, Jonathan D. Marmur, Kamel Khalili, Paul E. Klotman, Arnold Hampel, and Jay Rappaport
Optimization and Application of the Group I Ribozyme Trans-Splicing Reaction
Christer Einvik, Tonje Fiskaa, Eirik W. Lundblad, and Steinar Johansen
Repair of Myotonic Dystrophy Protein Kinase (DMPK) Transcripts by Trans-Splicing Ribozymes
Leonidas A. Phylactou
General Design and Construction of RNase P Ribozymes for Gene-Targeting Application
Hua Zou, Karen Chan, Phong Trang, and Fenyong Liu
In Vitro Selection of RNase P Ribozymes That Efficiently Cleave a Target mRNA
Kihoon Kim and Fenyong Liu
In Vitro
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