Ribozymes and siRNA protocols

著者

• Sioud, Mouldy

書誌事項

Ribozymes and siRNA protocols

edited by Mouldy Sioud

（Methods in molecular biology / John M. Walker, series editor, v. 252）

Humana Press, c2004

2nd ed.

注記

Includes bibliographical references and index

内容説明・目次

内容説明

During the last few years, new discoveries in the RNA field have opened up a wealth of opportunities to specifically target mRNA for functional analysis and target validation. Contained in this volume of Methods in Molecular Biology are methods useful for the design and application of ribozymes, DNAzymes, and small interfering RNAs (siRNAs). In addition, a number of illustrations aiming to facilitate the und- standing and application of each method is included. Like any new field, ribozymes and siRNA research is rapidly evolving, and so too are the methods used to study, select, express, and control their structure and in vivo biological activities. Ribozymes and siRNA Protocols, Second Edition focuses on the latest technical advances in interfering with gene expression. It contains a short int- duction followed by general and specific protocols for using hammerhead ribozymes and derivates, DNAzymes, hairpin ribozymes, group I intron ribozymes, RNase P ribozymes, and siRNAs. Also in this volume are general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy protocols using ribozymes.

目次

Ribozyme- and siRNA-Mediated mRNA Degradation: A General Introduction Mouldy Sioud Combination of Chemical and Enzymatic RNA Synthesis Rajesh K. Gaur, Andreas Hanne, and Guido Krupp Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes Martha J. Fedor Real-Time Monitoring of RNA and DNA Reactions by Fluorescence Detection Kumud K. Singh, Andreas Hanne, and Guido Krupp Quantification of Ribozyme Target RNA Using Real-Time PCR Dagmar Klein, Camillo Ricordi, and Ricardo L. Pastori Analysis of Ribozyme Structure and Function by Nucleotide Analog Interference Mapping Soumitra Basu, Catherine Pazsint, and Gopeswar Chowdhury Analysis of Global Conformational Transitions in Ribozymes David M. J. Lilley In Vivo Detection of Ribozyme Cleavage Products and RNA Structure by Use of Terminal Transferase-Dependent PCR Hsiu-Hua Chen, Daniela Castanotto, Jeanne LeBon, John J. Rossi, and Arthur D. Riggs Identification of Efficient Cleavage Sites in Long-Target RNAs Wei-Hua Pan and Gary A. Clawson Selection In Vitro of Allosteric Ribozymes Adam Roth and Ronald R. Breaker Regulation of Ribozyme Cleavage Activity by Oligonucleotides Yasuo Komatsu and Eiko Ohtsuka Tetracycline-Regulated Expression of Hammerhead Ribozymes In Vivo Emma T. Bowden and Anna T. Riegel Ribozyme Expression Systems Masayuki Sano and Kazunari Taira Design and Expression of Chimeric U1/Ribozyme Transgenes Roger Abounader, Robert Montgomery, Harry Dietz, and John Laterra Design and Validation of Therapeutic Hammerhead Ribozymes for Autosomal Dominant Diseases Jason J. Fritz, Marina Gorbatyuk, Alfred S. Lewin, and William W. Hauswirth Helicase-Attached Novel Hybrid Ribozymes Hiroaki Kawasaki, Masaki Warashina, Tomoko Kuwabara, and Kazunari Taira Functional Gene Discovery Using Hybrid Ribozyme Libraries Yoshio Kato, Masaru Tsunemi, Makoto Miyagishi, Hiroaki Kawasaki, and Kazunari Taira Maxizyme Technology Mayu Iyo, Hiroaki Kawasaki, and Kazunari Taira Target-Site Selection for the 10-23 DNAzyme Murray J. Cairns and Lun-Quan Sun In Vitro Selected RNA-Cleaving DNA Enzymes From Combinatorial Libraries Samitabh Chakraborti, Bandi Sriram and Akhil C. Banerjea Nucleic Acid Sequence Analysis Using DNAzymes Murray J. Cairns and Lun-Quan Sun Crystallization of the Hairpin Ribozyme: Illustrative Protocols Peter B. Rupert and Adrian R. Ferre-D'Amare An Experimental Method for Selecting Effective Target Sites and Designing Hairpin Ribozymes Alicia Barroso-delJesus, Elena Puerta-Fernandez, Cristina Romero-Lopez, and Alfredo Berzal-Herranz Design and Optimization of Sequence-Specific Hairpin Ribozymes Cristina Romero-Lopez, Alicia Barroso-delJesus, Elena Puerta-Fernandez, and Alfredo Berzal-Herranz Design, Targeting, and Initial Screening of sTRSV-Derived Hairpin Ribozymes for Optimum Helix 1 Length and Catalytic Efficiency In Vitro Max W. Richardson, Linda Hostalek, Michelle Dobson, Jason Hu, Richard Shippy, Andrew Siwkowski, Jonathan D. Marmur, Kamel Khalili, Paul E. Klotman, Arnold Hampel, and Jay Rappaport Optimization and Application of the Group I Ribozyme Trans-Splicing Reaction Christer Einvik, Tonje Fiskaa, Eirik W. Lundblad, and Steinar Johansen Repair of Myotonic Dystrophy Protein Kinase (DMPK) Transcripts by Trans-Splicing Ribozymes Leonidas A. Phylactou General Design and Construction of RNase P Ribozymes for Gene-Targeting Application Hua Zou, Karen Chan, Phong Trang, and Fenyong Liu In Vitro Selection of RNase P Ribozymes That Efficiently Cleave a Target mRNA Kihoon Kim and Fenyong Liu In Vitro

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詳細情報

• NII書誌ID(NCID): BA7008002X
• ISBN:
  • 1588292266
• LCCN: 2003016680
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Totowa, N.J.
• ページ数/冊数: xv, 623 p.
• 大きさ: 24 cm.
• 分類:
  • LCC : QP623.5.C36
  • NLM : W1
  • NLM : QU 25
  • DC22 : 572.8/8
• 件名:
  • LCSH : Catalytic RNA -- Laboratory manuals
  • LCSH : Small interfering RNA -- Laboratory manuals
  • MESH : RNA, Catalytic -- Laboratory Manuals
  • MESH : Gene Expression Profiling -- Laboratory Manuals
  • MESH : Gene Therapy -- Laboratory Manuals
  • MESH : RNA, Small Interfering -- Laboratory Manuals
• 親書誌ID:
  • BA10794673