Apoptosis methods and protocols
Author(s)
Bibliographic Information
Apoptosis methods and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 282）
Humana Press, c2004
- (alk. paper)
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Note
Includes bibliographical references and index
e-ISBN :1592598129
Description and Table of Contents
Description
This is a collection of cutting-edge techniques for detecting and quantifying apoptosis, understanding its biochemistry, and for identifying the genes and proteins that regulate and carry it out. Described in step-by-step detail, these readily reproducible methods range from flow cytometry and immunohistochemical procedures to kinase activity assays, yeast two-hybrid screening, and the cloning of novel genes by differential expression. The protocols follow the successful "Methods in Molecular Biology" series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. "Apoptosis Methods and Protocols" constitutes a key technical reference to the significant methodologies used in the field, as well as offering novice and experienced researchers alike powerful tools to illuminate the phenomenon of programmed cell death.
Table of Contents
Measurement of Apoptosis by DNA Fragmentation Demetrius Matassov, Terri Kagan, Julie Leblanc, Marianna Sikorska, and Zahra Zakeri Measurement of Caspase Activity in Cells Undergoing Apoptosis Sharad Kumar Flow Cytometry-Based Methods for Apoptosis Detection in Lymphoid Cells Owen Williams Methods of Analyzing Chromatin Changes Accompanying Apoptosis of Target Cells in Killer Cell Assays Richard C. Duke Detecting and Quantifying Apoptosis in Tissue Sections Vicki Save, Peter A. Hall, and Philip J. Coates Immunoassay for Single-Stranded DNA in Apoptotic Cells Oskar S. Frankfurt Methods to Measure Membrane Potential and Permeability Transition in Mitochondria During Apoptosis Naoufal Zamzami and Guido Kroemer Measurement of Changes in Intracellular Calcium During Apoptosis David J. McConkey and Leta Nutt Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2 Gabriel Gil-Gomez Assays to Measure Stress-Activated MAPK Activity Carme Caelles and Monica Morales Methods for Culturing Primary Sympathetic Neurons and for Determining Neuronal Viability Jonathan Whitfield, Stephen J. Neame, and Jonathan Ham Immunocytochemical Techniques for Studying Apoptosis in Primary Sympathetic Neurons Stephen J. Neame, Jonathan Whitfield, and Jonathan Ham Measuring Programmed Cell Death in Plants Ludmila Rizhsky, Vladimir Shulaev, and Ron Mittler Detection of Apoptosis in Drosophila Kimberly McCall and Jeanne S. Peterson Measurement of Apoptotic Cell Clearance In Vitro Andrew Devitt and Christopher D. Gregory Yeast Two-Hybrid Screening for Proteins Interacting With the Anti-Apoptotic Protein A20 Karen Heyninck, Sofie Van Huffel, Marja Kreike, and Rudi Beyaert The Yeast Three-Hybrid System As a Tool to Study Caspases Wim van Criekinge, Peter Schotte, Karen Heyninck, and Rudi Beyaert Cloning of Apoptosis-Related Genes by Representational Difference Analysis of cDNA Michael Hubank, Fredrik Bryntesson, Jennifer Regan, and David G. Schatz Identification of Apoptosis Regulatory Genes Using Insertional Mutagenesis Joelle Thomas, Yann Leverrier, Anne-Laure Mathieu, and Jacqueline Marvel Index
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