Pancreatic cancer : methods and protocols
著者
書誌事項
Pancreatic cancer : methods and protocols
(Methods in molecular medicine / John M. Walker, series editor, 103)
Humana Press, c2005
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Pancreatic ductal adenocarcinoma is the fourth leading cause of cancer death in the United States. Annually approximately 30,000 Americans are diagnosed with the disease and most will die from it within five years. P- creatic ductal adenocarcinoma is unique because of its late onset in age, high mortality, small tumor samples infiltrated with normal cells, and a lack of both early detection and effective therapies. Some of these characteristics have made studying this disease a challenge. Pancreatic cancer develops as a result of the accumulation of genetic alterations in cancer-causing genes, such as the oncogenes and the tumor-s- pressor genes. In the last decade, major progress has been made in identifying important oncogenes and tumor-suppressor genes for the disease. In Panc- atic Cancer: Methods and Protocols, we review the classical techniques that have contributed to the advances in pancreatic research and introduce new strategies that we hope will add to future breakthroughs in the field of cancer biology. Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of s- radic and familial pancreatic cancer. It covers topics from in vitro cell c- tures to in vivo mouse models, DNA to protein manipulation, and mutation analyses to treatment development. We believe that our book will prove an invaluable source of proven protocols for those who are interested in either basic or translational research in pancreatic cancer.
目次
Identification and Analysis of Precursors to Invasive Pancreatic Cancer
Ralph H. Hruban, Robb E. Wilentz, and Anirban Maitra
Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications
G. Steven Bova, Isam A. Eltoum, John A. Kiernan, Gene P. Siegal, Andra R. Frost, Carolyn J. M. Best, John W. Gillespie, and Michael R. Emmert-Buck
Immunohistochemistry and In Situ Hybridization in Pancreatic Neoplasia
Robb E. Wilentz, Ayman Rahman, Pedram Argani, and Christine Iacobuzio-Donahue
Practical Methods for Tissue Microarray Construction
Helen L. Fedor and Angelo M. De Marzo
Xenografting and Harvesting Human Ductal Pancreatic Adenocarcinomas for DNA Analysis
Kimberly Walter, James Eshleman, and Michael Goggins
Culture and Immortalization of Pancreatic Ductal Epithelial Cells
Terence Lawson, Michel Ouellette, Carol Kolar, and Michael Hollingsworth
DNA Methylation Analysis in Human Cancer
Carmelle D. Curtis and Michael Goggins
Digital Single-Nucleotide Polymorphism Analysis for Allelic Imbalance
Hsueh-Wei Chang and Ie-Ming Shih
Representational Difference Analysis as a Tool in the Search for New Tumor Suppressor Genes
Antoinette Hollestelle and Mieke Schutte
Serial Analyses of Gene Expression (SAGE)
Jia Le Dai
Oligonucleotide-Directed Microarray Gene Profiling of Pancreatic Adenocarcinoma
David E. Misek, Rork Kuick, Samir M. Hanash, and Craig D. Logsdon
Identification of Differentially Expressed Proteins in Pancreatic Cancer Using a Global Proteomic Approach
Christophe Rosty and Michael Goggins
Detection of Telomerase Activity in Patients with Pancreatic Cancer
Kazuhiro Mizumoto and Masao Tanaka
Serological Analysis of Expression cDNA Libraries (SEREX): An Immunoscreening Technique for Identifying Immunogenic Tumor Antigens
Yao-Tseng Chen, Ali O. Gure, and Matthew J. Scanlan
Modeling Pancreatic Cancer in Animals to Address Specific Hypotheses
Paul J. Grippo and Eric P. Sandgren
Strategies for the Use of Site-Specific Recombinases in Genome Engineering
Julie R. Jones, Kathy D. Shelton, and Mark A. Magnuson
Primary Explant Cultures of Adult and Embryonic Pancreas
Farzad Esni, Yoshiharu Miyamoto, Steven D. Leach, and Bidyut Ghosh
Zebrafish as a Model for Pancreatic Cancer Research
Nelson S. Yee and Michael Pack
Development of a Cytokine-Modified Allogeneic Whole Cell Pancreatic Cancer Vaccine
Dan Laheru, Barbara Biedrzycki, Amy M. Thomas, and Elizabeth M. Jaffee
Overview of Linkage Analysis: Application to Pancreatic Cancer
Alison P. Klein
Index
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