Cell migration : developmental methods and protocols
著者
書誌事項
Cell migration : developmental methods and protocols
(Methods in molecular biology / John M. Walker, series editor, v. 294)
Humana Press, c2005
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
目次
Part I. Introduction
Cell Migration: An Overview
Donna J. Webb, Huaye Zhang, and Alan F. Horwitz
Part II. Basic Cell Migration and Related Assays
Boyden Chamber Assay
Hong-Chen Chen
Wound-Healing Assay
Luis G. Rodriguez, Xiaoyang Wu, and Jun-Lin Guan
Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse Microscopy
Claire M. Wells and Anne J. Ridley
Cell-Adhesion Assays
Dennis F. Kucik and Chuanyue Wu
Cell-Spreading Assays
Allison L. Berrier and Susan E. LaFlamme
Cell-Scatter Assay
Hong-Chen Chen
Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices
Edna Cukierman
Part III. Analysis of Cell Migration in Specialized Cell Systems
Tumor Cell Invasion Assays
Leslie M. Shaw
Analysis of Endothelial Cell Migration Under Flow
Song Li
Angiogenesis Assays in the Chick CAM
Chris Storgard, David Mikolon, and Dwayne G. Stupack
Investigations of Neuronal Migration in the Central Nervous System
Michael E. Ward and Yi Rao
Part IV. Analysis of Cell Migration in Model Organisms
Analysis of Cell Migration in Caenorhabditis elegans
M. Afaq Shakir and Erik A. Lundquist
Analysis of Cell Migration Using Drosophila as a Model System
Jocelyn A. McDonald and Denise J. Montell
Imaging Cell Movement During Dorsal Closure in Drosophila Embryos
William Wood and Antonio Jacinto
Analysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cell Populations In Vivo
Diane S. Sepich and Lilianna Solnica-Krezel
The Xenopus Embryo as a Model System for Studies of Cell Migration
Douglas W. DeSimone, Lance Davidson, Mungo Marsden, and Dominique Alfandari
Neural Crest Migration Methods in the Chicken Embryo
Maria Elena de Bellard and Marianne Bronner-Fraser
In Vivo and In Vitro Models of Mammalian Angiogenesis
Mien V. Hoang and Donald R. Senger
Part V. Biochemical and Novel Approaches in Cell Migration
Measurement of Protein Tyrosine Phosphorylation in Cell Adhesion
Vidhya V. Iyer, Christoph Ballestrem, Joachim Kirchner, Benjamin Geiger, and Michael D. Schaller
Cell-Adhesion Assays: Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Membrane Patches
Frauke Drees, Amy Reilein, and W. James Nelson
Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cells
Christoph Ballestrem and Benjamin Geiger
Use of Multiphoton Imaging for Studying Cell Migration in the Mouse
Andrea Flesken-Nikitin, Rebecca M. Williams, Warren R. Zipfel, Watt W. Webb, and Alexander Yu. Nikitin
Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration
Daniel S. Rhoads, Sharvari M. Nadkarni, Loling Song, Camilla Voeltz, Eberhard Bodenschatz, and Jun-Lin Guan
Index
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