Capillary electrophoresis of proteins and peptides

Author(s)

• Strege, Mark A.
• Lagu, Avinash L.

Bibliographic Information

Capillary electrophoresis of proteins and peptides

edited by Mark A. Strege, Avinash L. Lagu

（Methods in molecular biology / John M. Walker, series editor, v. 276）

Humana Press, c2004

Note

Includes bibliographical references and index

Contents of Works

• Surfactant based methods for prevention of protein adsorption in capillary electrophoresis / Charles A. Lucy, Nicole E. Baryla, and Ken K.-C. Yeung
• Capillary coating for protein separation based on Si-O and Si-C covalent bond formation for capillary electrophoresis with laser-induced fluorescence detection / Hossein Ahmadzadeh, Norman J. Dovichi, and Sergey Krylov
• On-column labeling reaction for the analysis of protein contents of a single cell using capillary electrophoresis with laser-induced fluorescence detection / Hossein Ahmadzadeh and Sergey Krylov
• Covalent and noncovalent labeling schemes for near infrared dyes in capillary electrophoresis protein applications / John Sowell ... [et al.]
• Capillary electrophoresis in the analysis and monitoring of biotechnological processes / Vadim Klyushnichenko
• Capillary electrophoresis of proteins in a quality control environment / David L. Good ... [et al.]
• Analysis of neutral N-linked oligosaccharides from antibodies using free-solution capillary electrophoresis in bare fused silica capillaries / Jeffrey S. Patrick ... [et al.]
• Affinity capillary electrophoresis to examine receptor-ligand interactions / Maryam Azad ... [et al.]
• Screening major binding sites on human serum albumin by affinity capillary electrophoresis / Hee Seung Kim, John Austin, and David S. Hage
• Using charge ladders and capillary electrophoresis to measure the charge, size, and electrostatic interactions of proteins / Upma Sharma and Jeffrey D. Carbeck
• Frontal analysis continuous capillary electrophoresis for protein-polyelectrolyte binding studies / Emek Seyrek, Toshiaki Hattori, and Paul L. Dubin
• Analysis of proteins by CE, CIEF, and microfluidic devices with whole column imaging detection / Jiaqi Wu ... [et al.]
• Capillary electrophoresis electrospray ionization mass spectrometry of amino acids, peptides, and proteins / Mehdi Moini
• Capillary isoelectric focusing - mass spectrometry of proteins and protein complexes / Suzana Martinović, Ljiljana Paša-Tolić, and Richard D. Smith
• Integrated system for rapid proteomics analyses using microfluidics devices coupled to nanoelectrospray mass spectrometry / Jianjun Li ... [et al.]

Description and Table of Contents

Description

Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1-4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5-7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.

Table of Contents

Surfactant-Based Methods for Prevention of Protein Adsorption in Capillary Electrophoresis Charles A. Lucy, Nicole E. Baryla, and Ken K.-C. Yeung Capillary Coating for Protein Separation Based on Si-O and Si-C Covalent Bond Formation for Capillary Electrophoresis With Laser-Induced Fluorescence Detection Hossein Ahmadzadeh, Norman J. Dovichi, and Sergey Krylov On-Column Labeling Reaction for Analysis of Protein Contents of a Single Cell Using Capillary Electrophoresis With Laser-Induced Fluorescence Detection Hossein Ahmadzadeh and Sergey Krylov Covalent and Noncovalent Labeling Schemes for Near-Infrared Dyes in Capillary Electrophoresis Protein Applications John Sowell, Jozef Salon, Lucjan Strekowski, and Gabor Patonay Capillary Electrophoresis in the Analysis and Monitoring of Biotechnological Processes Vadim Klyushnichenko Capillary Electrophoresis of Proteins in a Quality Control Environment David L. Good, Stacey Cummins-Bitz, Raeann M. Fields, and Brian K. Nunnally Analysis of Neutral N-Linked Oligosaccharides From Antibodies Using Free-Solution Capillary Electrophoresis in Bare Fused-Silica Capillaries Jeffrey S. Patrick, Brenda P. Rener, Gregory S. Clanton, and Avinash L. Lagu Affinity Capillary Electrophoresis to Examine Receptor-Ligand Interactions Maryam Azad, John Kaddis, Valerie Villareal, Lili Hernandez, Catherine Silverio, and Frank A. Gomez Screening Major Binding Sites on Human Serum Albumin by Affinity Capillary Electrophoresis Hee Seung Kim, John Austin, and David S. Hage Using Charge Ladders and Capillary Electrophoresis to Measure the Charge, Size, and Electrostatic Interactions of Proteins Upma Sharma and Jeffrey D. Carbeck Frontal Analysis Continuous Capillary Electrophoresis for Protein-Polyelectrolyte Binding Studies Emek Seyrek, Toshiaki Hattori, and Paul L. Dubin Analysis of Proteins by CE, CIEF, and Microfluidic Devices With Whole-Column-Imaging Detection Jiaqi Wu, Xing-Zheng Wu, Tiemin Huang, and Janusz Pawliszyn Capillary Electrophoresis-Electrospray Ionization Mass Spectrometry of Amino Acids, Peptides, and Proteins Mehdi Moini Capillary Isoelectric Focusing-Mass Spectrometry of Proteins and Protein Complexes Suzana Martinovic, Ljiljana Pasa-Tolic, and Richard D. Smith Integrated System for Rapid Proteomics Analyses Using Microfluidic Devices Coupled to Nanoelectrospray Mass Spectrometry Jianjun Li, Tammy-Lynn Tremblay, Jed Harrison, and Pierre Thibault Index

by "Nielsen BookData"

Details

• NCID: BA70594118
• ISBN:
  • 1588290174
• LCCN: 2003028088
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Totowa, N.J.
• Pages/Volumes: xi, 332 p.
• Size: 24 cm.
• Classification:
  • LCC : QP519.9.C36
  • NLM : W1
  • NLM : QU 25
  • DC22 : 572/.636
• Subject Headings:
  • LCSH : Capillary electrophoresis -- Laboratory manuals
  • LCSH : Proteins -- Separation -- Laboratory manuals
  • LCSH : Peptides -- Separation -- Laboratory manuals
  • MESH : Electrophoresis, Capillary -- methods
  • MESH : Proteins -- analysis
• Parent Bibliography ID:
  • BA10794673