Genetic recombination : reviews and protocols
著者
書誌事項
Genetic recombination : reviews and protocols
(Methods in molecular biology / John M. Walker, series editor, v. 262)
Humana Press, c2004
大学図書館所蔵 全6件
  青森
  岩手
  宮城
  秋田
  山形
  福島
  茨城
  栃木
  群馬
  埼玉
  千葉
  東京
  神奈川
  新潟
  富山
  石川
  福井
  山梨
  長野
  岐阜
  静岡
  愛知
  三重
  滋賀
  京都
  大阪
  兵庫
  奈良
  和歌山
  鳥取
  島根
  岡山
  広島
  山口
  徳島
  香川
  愛媛
  高知
  福岡
  佐賀
  長崎
  熊本
  大分
  宮崎
  鹿児島
  沖縄
  韓国
  中国
  タイ
  イギリス
  ドイツ
  スイス
  フランス
  ベルギー
  オランダ
  スウェーデン
  ノルウェー
  アメリカ
注記
Includes bibliographical references and index
内容説明・目次
内容説明
Genetic recombination, in the broadest sense, can be defined as any process in which DNA sequences interact and undergo a transfer of information, producing new "recombinant" sequences that contain information from each of the original molecules. All organisms have the ability to carry out recombination, and this striking universality speaks to the essential role recombination plays in a variety of biological processes fundamentally important to the maintenance of life. Such processes include DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. One important aspect of recombination is that it typically occurs only between sequences that display a high degree of sequence identity. The stringent requirement for homology helps to ensure that, under normal circumstances, a cell is protected from deleterious rearrangements since a swap of genetic information between two nearly identical sequences is not expected to dramatically alter a genome. Recombination between dissimilar sequences, which does happen on occasion, may have such harmful consequences as chromosomal translocations, deletions, or inversions. For many organisms, it is also important that recombination rates are not too high lest the genome become destabilized. Curiously, certain organisms, such as the trypanosome parasite, actually use a high rate of recombination at a particular locus in order to switch antigen expression continually and evade the host immune system effectively.
目次
Part I. Studying Recombination Events in Eukaryotes
Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae
Rachelle Miller Spell and Sue Jinks-Robertson
Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells
Jason A. Smith and Alan S. Waldman
Intrachromosomal Homologous Recombination in Arabidopsis thaliana
Waltraud Schmidt-Puchta, Nadiya Orel, Anzhela Kyryk, and Holger Puchta
Analysis of Recombinational Repair of DNA Double-Strand Breaks in Mammalian Cells With I-SceI Nuclease
Jac A. Nickoloff and Mark A. Brenneman
Transformation of Monomorphic and Pleomorphic Trypanosoma brucei
Richard McCulloch, Erik Vassella, Peter Burton, Michael Boshart, and J. David Barry
Forward Genetic Screens for Meiotic and Mitotic Recombination-Defective Mutants in Mice
Laura Reinholdt, Terry Ashley, John Schimenti, and Naoko Shima
Part II. Recombination as a Reporter of Genomic Instability
Detecting Carcinogens With the Yeast DEL Assay
Richard J. Brennan and Robert H. Schiestl
In Vivo DNA Deletion Assay to Detect Environmental and Genetic Predisposition to Cancer
Ramune Reliene, Alexander J. R. Bishop, Jiri Aubrecht, and Robert H. Schiestl
Part III. Recombination as a Tool for Producing Targeted Genetic Modification
Gene Targeting at the Chromosomal Immunoglobulin Locus: A Model System for the Study of Mammalian Homologous Recombination Mechanisms
Mark D. Baker
DNA Fragment Transplacement in Saccharomyces cerevisiae: Some Genetic Considerations
Glenn M. Manthey, Michelle S. Navarro, and Adam M. Bailis
Targeted Gene Modification Using Triplex-Forming Oligonucleotides
Jean Y. Kuan and Peter M. Glazer
Using Nucleases to Stimulate Homologous Recombination
Dana Carroll
Enhancement of In Vivo Targeted Nucleotide Exchange by Nonspecific Carrier DNA
Katie K. Maguire and Eric B. Kmiec
Part IV. Biochemistry of Recombination
Chromatin Immunoprecipitation to Investigate Protein-DNA Interactions During Genetic Recombination
Tamara Goldfarb and Eric Alani
Holliday Junction Branch Migration and Resolution Assays
Angelos Constantinou and Stephen C. West
Index
「Nielsen BookData」 より