Genetic recombination : reviews and protocols
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Bibliographic Information
Genetic recombination : reviews and protocols
（Methods in molecular biology / John M. Walker, series editor, v. 262）
Humana Press, c2004
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Includes bibliographical references and index
Description and Table of Contents
Description
Genetic recombination, in the broadest sense, can be defined as any process in which DNA sequences interact and undergo a transfer of information, producing new "recombinant" sequences that contain information from each of the original molecules. All organisms have the ability to carry out recombination, and this striking universality speaks to the essential role recombination plays in a variety of biological processes fundamentally important to the maintenance of life. Such processes include DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. One important aspect of recombination is that it typically occurs only between sequences that display a high degree of sequence identity. The stringent requirement for homology helps to ensure that, under normal circumstances, a cell is protected from deleterious rearrangements since a swap of genetic information between two nearly identical sequences is not expected to dramatically alter a genome. Recombination between dissimilar sequences, which does happen on occasion, may have such harmful consequences as chromosomal translocations, deletions, or inversions. For many organisms, it is also important that recombination rates are not too high lest the genome become destabilized. Curiously, certain organisms, such as the trypanosome parasite, actually use a high rate of recombination at a particular locus in order to switch antigen expression continually and evade the host immune system effectively.
Table of Contents
Part I. Studying Recombination Events in Eukaryotes
Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae
Rachelle Miller Spell and Sue Jinks-Robertson
Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells
Jason A. Smith and Alan S. Waldman
Intrachromosomal Homologous Recombination in Arabidopsis thaliana
Waltraud Schmidt-Puchta, Nadiya Orel, Anzhela Kyryk, and Holger Puchta
Analysis of Recombinational Repair of DNA Double-Strand Breaks in Mammalian Cells With I-SceI Nuclease
Jac A. Nickoloff and Mark A. Brenneman
Transformation of Monomorphic and Pleomorphic Trypanosoma brucei
Richard McCulloch, Erik Vassella, Peter Burton, Michael Boshart, and J. David Barry
Forward Genetic Screens for Meiotic and Mitotic Recombination-Defective Mutants in Mice
Laura Reinholdt, Terry Ashley, John Schimenti, and Naoko Shima
Part II. Recombination as a Reporter of Genomic Instability
Detecting Carcinogens With the Yeast DEL Assay
Richard J. Brennan and Robert H. Schiestl
In Vivo DNA Deletion Assay to Detect Environmental and Genetic Predisposition to Cancer
Ramune Reliene, Alexander J. R. Bishop, Jiri Aubrecht, and Robert H. Schiestl
Part III. Recombination as a Tool for Producing Targeted Genetic Modification
Gene Targeting at the Chromosomal Immunoglobulin Locus: A Model System for the Study of Mammalian Homologous Recombination Mechanisms
Mark D. Baker
DNA Fragment Transplacement in Saccharomyces cerevisiae: Some Genetic Considerations
Glenn M. Manthey, Michelle S. Navarro, and Adam M. Bailis
Targeted Gene Modification Using Triplex-Forming Oligonucleotides
Jean Y. Kuan and Peter M. Glazer
Using Nucleases to Stimulate Homologous Recombination
Dana Carroll
Enhancement of In Vivo Targeted Nucleotide Exchange by Nonspecific Carrier DNA
Katie K. Maguire and Eric B. Kmiec
Part IV. Biochemistry of Recombination
Chromatin Immunoprecipitation to Investigate Protein-DNA Interactions During Genetic Recombination
Tamara Goldfarb and Eric Alani
Holliday Junction Branch Migration and Resolution Assays
Angelos Constantinou and Stephen C. West
Index
by "Nielsen BookData"
