mRNA processing and metabolism : methods and protocols

著者

• Schoenberg, Daniel R.

書誌事項

mRNA processing and metabolism : methods and protocols

edited by Daniel R. Schoenberg

（Methods in molecular biology / John M. Walker, series editor, v. 257）

Humana Press, c2004

注記

Includes bibliographical references and index

収録内容

• Using chromatin immunoprecipitation to map cotranscriptional mRNA processing in saccharomyces cerevisiae / Michael-Christopher Keogh and Stephen Buratowski
• Identifying phosphoCTD-associating proteins / Hemali P. Phatnani and Arno L. Greenleaf
• Imaging alternative splicing in living cells / Eric J. Wagner ... [et al.]
• Tobramycin affinity tag purification of spliceosomes / Klaus Hartmuth, Hans-Peter Vornlocher and Reinhard Lührmann
• Using single-strand conformational polymorphism gel electrophoresis to analyze mutually exclusive alternative splicing / Alicia M. Celotto and Brenton R. Graveley
• Methods for the analysis of adenosine-to-inosine editing in RNA / Zuo Zhang and Gordon G. Carmichael
• Assaying nuclear messenger RNA export in human cells / Bryan R. Cullen
• RNA unwinding assay for DExD/H-Box RNA helicases / Stephanie S. Tseng-Rogenski and Tien-Hsien Chang
• Approaches for monitoring nuclear translation / Francisco J. Iborra, Dean A. Jackson, and Peter R. Cook
• Immunopurification and analysis of protein and RNA components of mRNP in mammalian cells / Fabrice Lejeune and Lynne E. Maquat
• Gene expression analysis of messenger RNP complexes / Luiz O.F. Penalva, Scott A. Tenenbaum, and Jack D. Keene
• Using the λN peptide to tether proteins to RNAs / Julie Baron-Benhamou ... [et al.]
• An efficient system for cap- and poly(A)-dependent translation in vitro / Yuri V. Svitkin and Nahum Sonenberg
• A poly(A) tail-responsive in vitro system for cap- or IRES-driven translation from HeLa cells / Christian Thoma, Antje Ostareck-Lederer and Matthias W. Hentze
• Assessing messenger RNA decapping in cellular extracts / Naomi Bergman ... [et al.]
• Analysis of RNA exonucleolytic activities in cellular extracts / Devi Mukherjee ... [et al.]
• Application of ligation-mediated reverse transcription polymerase chain reaction to the identification of in vivo endonuclease-generated messenger RNA decay intermediates / Mark N. Hanson and Daniel R. Schoenberg
• Biochemical dissection of RNA silencing in plants / Guiliang Tang and Phillip D. Zamore
• RNA interference of mRNA processing factors in drosophila S2 cells / Alicia M. Celotto and Brenton R. Graveley
• RNA interference by short hairpin RNAs expressed in vertebrate cells / Gregory J. Hannon and Douglas S. Conklin

内容説明・目次

内容説明

Cells possess a wealth of posttranscriptional control mechanisms that impact on every conceivable aspect of the life of an mRNA. These processes are intimately intertwined in an almost baroque manner, where promoter context influences the recruitment of splicing factors, where the majority of pre-mRNAs undergo alternative splicing, and where proteins deposited during nuclear processing impact distal cytoplasmic processing, translation, and decay. If there is a unifying theme to mRNA Processing and Metabolism: Methods and Protocols, it is that mRNA processing and metabolism are integrated processes. Many of the techniques used to study mRNA have been described in a previous volume of this series (RNA-Protein Interaction Protocols, Susan Haynes, ed.) and specialized methods journals. In selecting topics for mRNA Processing and Metabolism: Methods and Protocols, I sought input on new and novel techniques and approaches that build on this foundation using technological advances in microscopy, whole genome sequencing, microarrays, mass spectrometry, fluorescent detection methodologies, and RNA interference. I have tried not to bias this book toward any single model organism, and approaches described in the various chapters use yeast, Drosophila, Xenopus, mice, plants, and cultured mammalian cells.

目次

Using Chromatin Immunoprecipitation to Map Cotranscriptional mRNA Processing in Saccharomyces cerevisiae Michael-Christopher Keogh and Stephen Buratowski Identifying PhosphoCTD-Associating Proteins Hemali P. Phatnani and Arno L. Greenleaf Imaging Alternative Splicing in Living Cells Eric J. Wagner, Andrea Baines, Todd Albrecht, Robert M. Brazas, and Mariano A. Garcia-Blanco Tobramycin Affinity Tag Purification of Spliceosomes Klaus Hartmuth, Hans-Peter Vornlocher, and Reinhard Luhrmann Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Alternative Splicing Alicia M. Celotto and Brenton R. Graveley Methods for the Analysis of Adenosine-to-Inosine Editing in RNA Zuo Zhang and Gordon G. Carmichael Assaying Nuclear Messenger RNA Export in Human Cells Bryan R. Cullen RNA Unwinding Assay for DExD/H-Box RNA Helicases Stephanie S. Tseng-Rogenski and Tien-Hsien Chang Approaches for Monitoring Nuclear Translation Francisco J. Iborra, Dean A. Jackson, and Peter R. Cook Immunopurification and Analysis of Protein and RNA Components of mRNP in Mammalian Cells Fabrice Lejeune and Lynne E. Maquat Gene Expression Analysis of Messenger RNP Complexes Luiz O. F. Penalva, Scott A. Tenenbaum, and Jack D. Keene Using the lN Peptide to Tether Proteins to RNAs Julie Baron-Benhamou, Niels H. Gehring, Andreas E. Kulozik, and Matthias W. Hentze An Efficient System for Cap- and Poly(A)-Dependent Translation In Vitro Yuri V. Svitkin and Nahum Sonenberg A Poly(A) Tail-Responsive In Vitro System for Cap- or IRES-Driven Translation From HeLa Cells Christian Thoma, Antje Ostareck-Lederer, and Matthias W. Hentze Assessing Messenger RNA Decapping in Cellular Extracts Naomi Bergman, Joseph Milone, Elizabeth J. Bates, MateuszOpyrchal, Vivian Bellofatto, and Jeffrey Wilusz Analysis of RNA Exonucleolytic Activities in Cellular Extracts Devi Mukherjee, David T. Fritz, Walter J. Kilpatrick, Min Gao, and Jeffrey Wilusz Application of Ligation-Mediated Reverse Transcription Polymerase Chain Reaction to the Identification of In Vivo Endonuclease-Generated Messenger RNA Decay Intermediates Mark N. Hanson and Daniel R. Schoenberg Biochemical Dissection of RNA Silencing in Plants Guiliang Tang and Phillip D. Zamore RNA Interference of mRNA Processing Factors in Drosophila S2 Cells Alicia M. Celotto and Brenton R. Graveley RNA Interference by Short Hairpin RNAs Expressed in Vertebrate Cells Gregory J. Hannon and Douglas S. Conklin Index

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詳細情報

• NII書誌ID(NCID): BA70594548
• ISBN:
  • 1588292258
• LCCN: 2003027588
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Totowa, N.J.
• ページ数/冊数: xv, 270 p.
• 大きさ: 24 cm.
• 分類:
  • LCC : QP623.5.M47
  • NLM : W1
  • NLM : QU 25
  • DC22 : 572.8/8
• 件名:
  • LCSH : Messenger RNA -- Laboratory manuals
  • MESH : RNA, Messenger -- metabolism
  • MESH : Genetic Techniques
• 親書誌ID:
  • BA10794673