Cell cycle control : mechanisms and protocols

Author(s)

• Humphrey, Tim (Tim Carter)
• Brooks, Gavin

Bibliographic Information

Cell cycle control : mechanisms and protocols

edited by Tim Humphrey, Gavin Brooks

（Methods in molecular biology / John M. Walker, series editor, v. 296）

Humana Press, c2005

Note

Includes bibliographical references and index

Contents of Works

• Cell cycle molecules and mechanisms of the budding and fission yeasts / Tim Humphrey and Amanda Pearce
• The plant cell cycle : an overview / John H. Doonan
• The C. elegans cell cycle : overview of molecules and mechanisms / Sander van den Heuvel
• Developmental control of growth and cell cycle progression in drosophila / Lisa Swanhart, Jeremy Kupsco, and Robert J. Duronio
• The xenopus cell cycle : an overview / Anna Philpott and P. Renee Yew
• The mammalian cell cycle : an overview / Jane V. Harper and Gavin Brooks
• Synchronization of cell populations in G1/S and G2/M phases of the cell cycle / Jane V. Harper
• Mapping origins of DNA replication in eukaryotes / Susan A. Gerbi
• In situ assay for analyzing the chromatin binding of proteins in fission yeast / Stephen E. Kearsey ... [et al.]
• Application of magnetic beads to purify cells transiently transfected with plasmids encoding short hairpin RNAs / Peter D. Adams
• The cell cycle and virus infection / Steven R. Emmett ... [et al.]
• Methods for preparation of proteins and protein complexes that regulate the eukaryotic cell cycle for structural studies / Julie Welburn and Jane Endicott
• E2F transcription factors and pRb pocket proteins in cell cycle progression / Ludger Hauck and Rüdiger von Harsdorf
• Forkhead (FOX) transcription factors and the cell cycle : measurement of DNA binding by foxO and foxM transcription factors / Katrina A. Bicknell
• Measurement of geminin activity in xenopus egg extracts / Thomas J. McGarry
• CDK-activating kinases : detection and activity measurements / Stéphane Larochelle and Robert P. Fisher
• Cyclins, cyclin-dependent kinases, and cyclin-dependent kinase inhibitors : detection methods and activity measurements / Gavin Brooks
• Measurement of wee kinase activity / Paul R. Mueller and Walter F. Leise III
• CDC25 dual-specificity protein phosphatases : detection and activity measurements / Sorab N. Dalal and Melanie Volkening
• Assaying cell cycle checkpoints : activity of the protein kinase Chk1 / Carmela Palermo and Nancy C. Walworth
• Polo-like kinase-1 : activity measurement and RNAi-mediated knockdown / Marcel A.T.M. van Vugt and René H. Medema
• The Ipl1/aurora kinase family : methods of inhibition and functional analysis in mammalian cells / Claire Ditchfield, Nicholas Keen, and Stephen S. Taylor
• Purification of the Ndc80 kinetochore subcomplex from xenopus eggs / Mark L. McCleland and P. Todd Stukenberg

Description and Table of Contents

Description

The fundamental question of how cells grow and divide has perplexed biologists since the development of the cell theory in the mid-19th century, when it was recognized by Virchow and others that "all cells come from cells." In recent years, considerable effort has been applied to the identification of the basic molecules and mechanisms that regulate the cell cycle in a number of different organisms. Such studies have led to the elucidation of the central paradigms that underpin eukaryotic cell cycle control, for which Lee Hartwell, Tim Hunt, and Paul Nurse were jointly awarded the Nobel Prize for Medicine and Physiology in 2001 in recognition of their seminal contributions to this field. The importance of understanding the fundamental mechanisms that modulate cell division has been reiterated by relatively recent discoveries of links between cell cycle control and DNA repair, growth, cellular metabolism, development, and cell death. This new phase of integrated cell cycle research provides further challenges and opportunities to the biological and medical worlds in applying these basic concepts to understanding the etiology of cancer and other proliferative diseases.

Table of Contents

Part I. Overviews of Cell Cycle Control in Different Organisms Cell Cycle Molecules and Mechanisms of the Budding and Fission Yeasts Tim Humphrey and Amanda Pearce The Plant Cell Cycle: An Overview John H. Doonan The C. elegans Cell Cycle: Overview of Molecules and Mechanisms Sander van den Heuvel Developmental Control of Growth and Cell Cycle Progression in Drosophila Lisa Swanhart, Jeremy Kupsco, and Robert J. Duronio The Xenopus Cell Cycle: An Overview Anna Philpott and P. Renee Yew The Mammalian Cell Cycle: An Overvew Jane V. Harper and Gavin Brooks Part II. Cell Cycle Study Methods and Techniques Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle Jane V. Harper Mapping Origins of DNA Replication in Eukaryotes Susan A. Gerbi In Situ Assay for Analyzing the Chromatin Binding of Proteins in Fission Yeast Stephen E. Kearsey, Lydia Brimage, Mandana Namdar, Emma Ralph, and Xiaowen Yang Application of Magnetic Beads to Purify Cells Transiently Transfected With Plasmids Encoding Short Hairpin RNAs Peter D. Adams The Cell Cycle and Virus Infection Stevan R. Emmett, Brian Dove, Laura Mahoney, Torsten Wurm, and Julian A. Hiscox Methods for Preparation of Proteins and Protein Complexes That Regulate the Eukaryotic Cell Cycle for Structural Studies Julie Welburn and Jane Endicott Part III. Detection and Measurement of Cell Cycle Molecules E2F Transcription Factors and pRb Pocket Proteins in Cell Cycle Progression Ludger Hauck and Rudiger von Harsdorf Forkhead (FOX) Transcription Factors and the Cell Cycle: Measurement of DNA Binding by FoxO and FoxM Transcription Factors Katrina A. Bicknell Measurement of Geminin Activity in Xenopus Egg Extracts Thomas J. McGarry CDK-ActivatingKinases: Detection and Activity Measurements Stephane Larochelle and Robert P. Fisher Cyclins, Cyclin-Dependent Kinases, and Cyclin-Dependent Kinase Inhibitors: Detection Methods and Activity Measurements Gavin Brooks Measurement of Wee Kinase Activity Paul R. Mueller and Walter F. Leise III CDC25 Dual-Specificity Protein Phosphatases: Detection and Activity Measurements Sorab N. Dalal and Melanie Volkening Assaying Cell Cycle Checkpoints: Activity of the Protein Kinase Chk1 Carmela Palermo and Nancy C. Walworth Polo-Like Kinase-1: Activity Measurement and RNAi-Mediated Knockdown Marcel A. T. M. van Vugt and Rene H. Medema The Ipl1/Aurora Kinase Family: Methods of Inhibition and Functional Analysis in Mammalian Cells Claire Ditchfield, Nicholas Keen, and Stephen S. Taylor Purification of the Ndc80 Kinetochore Subcomplex From Xenopus Eggs Mark L. McCleland and P. Todd Stukenberg Index

by "Nielsen BookData"

• NCID: BA70695534
• ISBN:
  • 1588291448
• LCCN: 2004008991
• Country Code: us
• Title Language Code: eng
• Text Language Code: eng
• Place of Publication: Totowa, N.J.
• Pages/Volumes: xii, 402 p.
• Size: 24 cm
• Classification:
  • LCC : QH605
  • NLM : W1
  • NLM : QH 605
  • DC22 : 571.8/4
• Subject Headings:
  • LCSH : Cell cycle -- Laboratory manuals
  • MESH : Cell Cycle -- physiology
  • MESH : Eukaryotic Cells -- physiology
  • MESH : Molecular Biology -- methods
• Parent Bibliography ID:
  • BA10794673