Cell cycle control and dysregulation protocols : cyclins, cyclin-dependent kinases, and other factors

著者

• Giordano, Antonio, MD
• Romano, Gaetano

書誌事項

Cell cycle control and dysregulation protocols : cyclins, cyclin-dependent kinases, and other factors

edited by Antonio Giordano, Gaetano Romano

（Methods in molecular biology / John M. Walker, series editor, v. 285）

Humana Press, c2004

注記

Includes bibliographical references and index

収録内容

• The biology of cyclins and cyclin-dependent protein kinases : an introduction / Lucio Miele
• In situ immunofluorescence analysis : immunofluorescence microscopy / Amjad Javed ... [et al.]
• In situ immunofluorescence analysis : analyzing RNA synthesis by 5-bromouridine-5'-triphosphate labeling / Amjad Javed ... [et al.]
• Immunofluorescence analysis using epitope-tagged proteins : in vitro system / Amjad Javed ... [et al.]
• Analysis of in vivo gene expression using epitope-tagged proteins / Amjad Javed ... [et al.]
• Chromatin immunoprecipitation / Amjad Javed ... [et al.]
• Protein-deoxyribonucleic acid interactions linked to gene expression : electrophoretic mobility shift assay / Amjad Javed ... [et al.]
• Protein-deoxyribonucleic acid interactions linked to gene expression : DNase I digestion / Amjad Javed ... [et al.]
• Protein-deoxyribonucleic acid interactions linked to gene expression : ligation-mediated polymerase chain reaction / Amjad Javed ... [et al.]
• Assays for cyclin-dependent kinase inhibitors / Adrian M. Senderowicz
• Protein degradation via the proteosome / Henry Hoff, Hong Zhang, and Christian Sell
• The transformed phenotype / Henry Hoff ... [et al.]
• A morphologic approach to detect apoptosis based on electron microscopy / Martyn K. White and Caterina Cinti
• Detection of apoptotic deoxyribonucleic acid break by in situ nick translation / Carmela Trimarchi ... [et al.]
• Induction of deoxyribonucleic acid damage by alkylating agents / Salvatore Cortellino ... [et al.]
• Induction of deoxyribonucleic acid damage by γ irradiation / Salvatore Cortellino, David P. Turner and Alfonso Bellacosa
• Ultraviolet irradiation of cells / David P. Turner, Anthony T. Yeung, and Alfonso Bellacosa
• Transient production of retroviral- and lentiviral-based vectors for the transduction of mammalian cells / Tiziana Tonini ... [et al.]
• Determination of functional viral titer by drug-resistance colony assay, expression of green fluorescent protein, and β-galactoside staining / Tiziana Tonini ... [et al.]
• Retroviral and lentiviral vector titration by the analysis of the activity of viral reverse transcriptase / Tiziana Tonini ... [et al.]
• Single and double colloidal gold labeling in postembedding immunoelectron microscopy / Nicoletta Zini ... [et al.]
• Multifluorescence labeling and colocalization analyses / Massimo Riccio ... [et al.]

内容説明・目次

内容説明

Cell Cycle Control and Dysregulation Protocols focuses on emerging methodologies for studying the cell cycle, kinases, and kinase inhibitors. It addresses the issue of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Because there are already many manuals and protocols available, along with commercial kits and reagents, a variety of the more common techniques have not been included in our book. The protocols described, based on rather sophisticated techniques for in vivo and in vitro studies, consist of molecular biology, biochemistry, and various types of immunoassays. Indeed, the authors have successfully accomplished an arduous task by presenting several topics in the simplest possible manner. We are confident that Cell Cycle Control and Dysregulation Protocols will facilitate and optimize the work of practical scientists involved in researching the cell cycle. We greatly acknowledge the extraordinary contribution of the authors in writing this book.

目次

Part I. Analysis of Cyclins and Cyclin-Dependent Kinases The Biology of Cyclins and Cyclin-Dependent Protein Kinases: An Introduction Lucio Miele In Situ Immunofluorescence Analysis: Immunofluorescence Microscopy Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein In Situ Immunofluorescence Analysis: Analyzing RNA Synthesis by 5-Bromouridine-5'-Triphosphate Labeling Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein Immunofluorescence Analysis Using Epitope-Tagged Proteins: In Vitro System Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein Analysis of In Vivo Gene Expression Using Epitope-Tagged Proteins Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein Chromatin Immunoprecipitation Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Electrophoretic Mobility Shift Assay AmjadJaved, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: DNase I Digestion Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Ligation-Mediated Polymerase Chain Reaction Amjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. Stein Assays for Cyclin-Dependent Kinase Inhibitors Adrian M. Senderowicz. Protein Degradation Via the Proteosome, Henry Hoff, Hong Zhang, and Christian Sell Part II. Analysis of the Factors Involved in Cell Cycle Deregulation The Transformed Phenotype Henry Hoff, Barbara Belletti, Hong Zhang, and Christian Sell A Morphologic Approach to Detect Apoptosis Based on Electron Microscopy Martyn K. White and Caterina Cinti Detection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick Translation Carmela Trimarchi, Dario La Sala, Alessandra Zamparelli, and Caterina Cinti Part III. Cellular Response to Deoxyribonucleic Acid Damage Induction of Deoxyribonucleic Acid Damage by Alkylating Agents Salvatore Cortellino, David P. Turner, Domenico Albino, and Alfonso Bellacosa Induction of Deoxyribonucleic Acid Damage by g Irradiation Salvatore Cortellino, David P. Turner and Alfonso

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詳細情報

• NII書誌ID(NCID): BA7073815X
• ISBN:
  • 0896039498
• LCCN: 2004006931
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Totowa, N.J.
• ページ数/冊数: xiii, 185 p.
• 大きさ: 24 cm.
• 分類:
  • LCC : QH605
  • NLM : W1
  • NLM : QU 55
  • DC22 : 571.8/4
• 件名:
  • LCSH : Cell cycle -- Laboratory manuals
  • LCSH : Cyclin-dependent kinases -- Laboratory manuals
  • LCSH : Cyclins -- Laboratory manuals
  • MESH : Cell Cycle Proteins -- genetics
  • MESH : Cyclin-Dependent Kinases
  • MESH : DNA
  • MESH : Fluorescent Antibody Technique
  • MESH : Gene Expression
• 親書誌ID:
  • BA10794673