RNA interference (RNAi) : nuts & bolts of RNAi technology
著者
書誌事項
RNA interference (RNAi) : nuts & bolts of RNAi technology
(The nuts & bolts series)
DNA Press, c2003
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内容説明・目次
内容説明
This book offers a comprehensive guide to RNAi experimentation and provides in depth coverage of the application of this technology in plants, flies and mammalian cells. The chapters reflect recent developments in this rapidly growing field.
目次
- Chapter 1 Targeted Gene Silencing in Plants Using RNA Interference
- Chapter 2 RNAi in Caenorhabditis elegans
- Chapter 3 Application and Analysis of RNAi in Drosophila Systems
- Chapter 4 Gene Silencing by Synthetic siRNA Duplexes in Mammalian Cell Culture
- Chapter 5 Strategies for Synthesizing Small Interfering RNA (siRNA)
- Chapter 6 Generating Long dsRNA, Individual siRNA and siRNA Cocktails In Vitro
- Chapter 7 Optimizing High Throughput RNAi-based Assays Using Transient Transfection of Synthetic siRNAs in Cultured Mammalian Cells
- Chapter 8 Subcellular Delivery and Detection of Small Interfering RNA
- Chapter 9 Downregulation of Cellular Genes by PCR Products Expressing siRNAs Mammalian Cells
- Chapter 10 Generating Adenoviruses for shRNA or siRNA Delivery
- Chapter 11 Achieving Stable, Heritable Gene Silencing in the Mouse
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