Molecular pathology methods and reviews
著者
書誌事項
Molecular pathology methods and reviews
(Methods in molecular medicine / John M. Walker, series editor, 74 . Lung cancer ; 1)
Humana Press, c2003
- タイトル別名
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Lung cancer
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
The Methods in Molecular Medicine series is intended as a resource for both novice and experienced investigators attempting to diversify their tech- logical base in research. Lung Cancer: Volume 1: Molecular Pathology Me- ods and Reviews presents an overview of the current status of assays employed to detect and characterize the multitude of pathologies that contribute to the development of this deadly disease. As with all volumes in the Methods in Molecular Medicine series, the reader should find that each methods-based chapter provides clear instructions for the performance of various protocols, supplemented by additional technical notes that provide valuable insight. These notes are designed to enable the reader to acquire the techniques described with a proficiency not easily achieved by re- ing standard method formats. No volume can exhaustively cover every aspect of biological research and there will be gaps in this endeavor that one or another research group will id- tify. Each section herein could readily be expanded into a book in its own right. However, I have sought to include a spectrum of techniques that should allow for the acquisition of key skills in each area covered.
目次
Part I. Introduction
Characteristic Genetic Alterations in Lung Cancer
Ignacio I. Wistuba and Adi F. Gazdar
Part II. Etiology and Classification of Lung Tumors
Clinical and Biological Relevance of Recently Defined Categories of Pulmonary Neoplasia
Edward Gabrielson
Molecular Epidemiology of Human Cancer Risk: Gene-Environment Interactions and p53 Mutation Spectrum in Human Lung Cancer
Kirsi Vahakangas
Neuroendocrine Phenotype of Small Cell Lung Cancer
Judy M. Coulson, Marta Ocejo-Garcia, and Penella J. Woll
AIDS-Associated Pulmonary Cancers
Kushagra Katariya and Richard J. Thurer
Part III. Molecular Abnormalities in Lung Cancer
A. Detection of Alterations in the Cell Cycle. Abrogation of the RB-p16 Tumor Suppressor Pathway in Human Lung Cancer
Joseph Geradts
Sensitive Detection of Hypermethylated p16INK4a Alleles in Exfoliative Tissue Material
Marcus Schuermann and Michael Kersting
B. Detection of Alterations in Signal Transduction Pathways
Role of Receptor Tyrosine Kinases in Lung Cancer
Gautam Maulik, Takashi Kijima, and Ravi Salgia
Relationship of EGFR Signal-Transduction Modulation by Tyrosine Kinase Inhibitors to Chemosensitivity and Programmed Cell Death in Lung Cancer Cell Lines
Wendong Lei, Patrick P. Koty, and Mark L. Levitt
Detection of the Transcripts and Proteins for the Transforming Growth Factor-b Isoforms and Receptors in Mouse Lung Tumorigenesis Using In Situ Hybridization and Immunohistochemistry in Paraffin-Embedded Tissue Sections
Sonia B. Jakowlew and Jennifer Mariano
Alterations in the Expression of Insulin-Like Growth Factors and Their Binding Proteins in Lung Cancer
Carrie M. Coleman and Adda Grimberg
Screening of Mutations in the ras Family of Oncogenes by Polymerase Chain Reaction-Based Ligase Chain Reaction
AlfredoMartinez, Teresa A. Lehman, Rama Modali, and James L. Mulshine
Assays for Raf-1 Kinase Phosphorylation and Activity in Human Small Cell Lung Cancer Cells
Rajani K. Ravi and Barry D. Nelkin
g-Glutamylcysteine Synthetase in Lung Cancer: Effect on Cell Viability
Kristiina Jarvinen, Ylermi Soini, and Vuokko L. Kinnula
Localization of Cyclooxygenase-2 Protein Expression in Lung Cancer Specimens by Immunohistochemical Analysis
Sarah A. Wardlaw and Thomas H. March
Non-RI Protocols for L-myc Allelotyping and Deletion Mapping of Chromosome 1p in Primary Lung Cancers
Keiko Hiyama, Ciro Mendoza, and Eiso Hiyama
C. Detection of Altered Cell Surface Markers
Intercellular Adhesion Molecules (ICAM-1, VCAM-1, and LFA-1) in Adenocarcinoma of Lung: Technical Aspects
Armando E. Fraire, Bruce A. Woda, Louis Savas, and Zhong Jiang
MUC1 Expression in Lung Cancer
Eva Szabo
Altered Surface Markers in Lung Cancer: Lack of Cell-Surface Fas/APO-1 Expression in Pulmonary Adenocarcinoma May Allow Escape from Immune Surveillance
Yoshihiro Nambu and David G. Beer
D. Detection of Alterations in the Apoptotic Pathway
Prognostic Relevance of Angiogenic, Proliferative, and Apoptotic Factors in Lung Carcinomas: A Case Review
Manfred Volm and Reet Koomagi
Morphological Assessment of Apoptosis in Human Lung Cells
Lori D. Dwyer-Nield, David Dinsdale, J. John Cohen, Margaret K. T. Squier, and Alvin M. Malkinson
[D-Arg6, D-Trp7,9, NmePhe8]-Substance P (6-11) Activates JNK and Induces Apoptosis in Small Cell Lung Cancer Cells via an Oxidant-Dependent Mechanism
Alison MacKinnon and Tariq Sethi
Isolation of Cells Entering Different Programmed Cell Death Pathways Using a Discontinuous Percoll Gradient
Patrick P. Koty, Haifan Zhang, Wendong Lei, and Mark L. Levitt
E. Detection of Alterations in Lung
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