Principles and techniques of biochemistry and molecular biology

書誌事項

Principles and techniques of biochemistry and molecular biology

edited by Keith Wilson and John Walker

Cambridge University Press, 2005

6th ed

  • : hbk
  • : pbk

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注記

New & expanded ed. of: Principles and techniques of practical biochemistry. 5th ed. c2000

Includes bibliographical references and index

内容説明・目次

内容説明

This is a new and expanded edition of the bestselling Principles and Techniques of Practical Biochemistry. It includes discussion of the theoretical principles, practical details and applications of the key experimental techniques that are routinely used in modern biochemistry and molecular biology, including those that have led to the emergence of the new disciplines of genomics, proteomics and bioinformatics. All chapters have been updated and new sections added to cover the principles of experimental design, the statistical analysis of quantitative analytical data, and the principles and practice of clinical biochemistry. here are new chapters covering cell culture, microscopy and mass spectrometry. To enhance student understanding of each topic, in-text worked examples are included in each chapter. The textbook will be essential reading for all bioscience students and pre-clinical medical students.

目次

  • Preface
  • List of contributors
  • List of abbreviations
  • 1. Basic priniciples Keith Wilson and John Fyffe
  • 2. Cell culture techniques Anwar R. Baydoun
  • 3. Centrifugation Kay Ohlendieck
  • 4. Microscopy Stephen W. Paddock
  • 5. Molecular biology, bioinformatics and basic techniques Ralph Rapley
  • 6. Recombinant DNA and genetic analysis Ralph Rapley
  • 7. Immunochemical techniques Robin Thorpe and Susan Thorpe
  • 8. Protein structure, purification, characterisation and functional analysis John Walker
  • 9. Mass spectrometric techniques Alistair Aitken
  • 10. Electrophoretic techniques John Walker
  • 11. Chromatographic techniques Keith Wilson
  • 12. Spectroscopic techniques I: atomic and molecular electronic spectroscopy Derek B. Gordon
  • 13. Spectroscopic techniques II: vibrational spectroscopy and electron and nuclear spin orientation in magnetic fields
  • 14. Radioisotope techniques Robert J. Slater
  • 15. Enzymes Keith Wilson
  • 16. Cell membrane receptors and cell signalling Keith Wilson.

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