Human cell culture protocols
著者
書誌事項
Human cell culture protocols
(Methods in molecular medicine / John M. Walker, series editor, 107)
Humana Press, c2005
2nd ed. / edited by Joanna Picot
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
目次
Establishment and Maintenance of Normal Human Keratinocyte Cultures
Claire Linge
Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters
Mei-Yu Hsu, Ling Li, and Meenhard Herlyn
Isolation and Culture of Human Osteoblasts
Alison Gartland, Katherine A. Buckley, Jane P. Dillon, Judith M. Curran, John A. Hunt, and James A. Gallagher
Human Osteoclast Culture from Peripheral Blood Monocytes: Phenotypic Characterization and Quantitation of Resorption
Katherine A. Buckley, Benjamin Y. Y. Chan, William D. Fraser, and James A. Gallager
Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation
Mary B. Goldring
Human Myoblasts and Muscle-Derived SP Cells
Grace K. Pavlath and Emanuela Gussoni
Cell Cultures of Autopsy-Derived Fibroblasts
Volker Meske, Frank Albert, and Thomas G. Ohm
Primary Culture and Differentiation of Human Adipocyte Precursor Cells
Vanessa van Harmelen, Thomas Skurk, and Hans Hauner
Human Mononuclear Phagocytes in Tissue Culture
Yona Keisari
Purification of Peripheral Blood Natural Killer Cells
Bice Perussia and Matthew J. Loza
Human Fetal Brain Cell Culture
Mark P. Mattson
Culturing Human Schwann Cells
Victor J. Turnbull
Well-Differentiated Human Airway Epithelial Cell Cultures
M. Leslie Fulcher, Sherif Gabriel, Kimberlie A. Burns, James R. Yankaskas, and Scott H. Randell
Isolation and Culture of Human Alveolar Epithelial Cells
Carsten Ehrhardt, Kwang-Jin Kim, and Claus-Michael Lehr
A New Approach to Primary Culture of Human Gastric Epithelium
Pierre Chailler and Daniel Menard
Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach
Hamid A. Mohammadpour
Isolation and Culture of HumanHepatocytes
Martin Bayliss and Graham Somers
Glomerular Epithelial and Mesangial Cell Culture and Characterization
Heather M. Wilson and Keith N. Stewart
Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules
Gabrielle M. Hawksworth
Culture of Parathyroid Cells
Per Hellman
Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media
Daniel W. Fraga, A. Osama Gaber, and Malak Kotb
Primary Culture of Human Antral Endocrine and Epithelial Cells
Susan B. Curtis and Alison M. J. Buchan
Conjunctiva Organ and Cell Culture
Monica Berry and Marcus Radburn-Smith
Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium
Martin J. Stevens, Dennis D. Larkin, Eva L. Feldman, Monte A. DelMonte, and Douglas A. Greene
Index
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