Xenopus protocols : cell biology and signal transduction
著者
書誌事項
Xenopus protocols : cell biology and signal transduction
（Methods in molecular biology / John M. Walker, series editor, v. 322）
Humana Press, c2006
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
A collection of standard and cutting-edge techniques for using Xenopus oocytes and oocytes/egg extracts to reconstitute biological and cellular processes. These readily reproducible methods take advantage of the oocyte's impressive protein abundance, its striking protein translation capacity, and its breathtaking possibilities for the assembly of infectious viral particles by single cell injection of multiple RNAs. The authors focus on the versatility of frog oocytes and egg extracts in cell biology and signal transduction, and cover all the major uses of oocytes/extracts as experimental models.
目次
Resources for Genetic and Genomic Studies of Xenopus
Steven L. Klein, Daniela S. Gerhard, Lukas Wagner, Paul Richardson, Lynn M. Schriml, Amy K. Sater, Wesley C. Warren, and John D. McPherson
The Physiology of the Xenopus laevis Ovary
Melissa A. Rasar and Stephen R. Hammes
Oocyte Isolation and Enucleation
X. Shawn Liu and X. Johne Liu
Xenopus tropicalis Oocytes: More Than Just a Beautiful Genome
Jean-Francois L. Bodart and Nicholas S. Duesbery
Oocyte Expression With Injection of Purified T7 RNA Polymerase
Xavier Altafaj, Nathalie Joux, Michel Ronjat, and Michel De Waard
Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microscopy
Bret E. Becker and David L. Gard
Multiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte Research
Angela M. Prouty, Jun Wu, Da-Ting Lin, Patricia Camacho, and James D. Lechleiter
Imaging Ca2+ Signals in Xenopus Oocytes
Sheila L. Dargan, Angelo Demuro, and Ian Parker
Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs
Antonin V. Tutter and Johannes C. Walter
Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes
Daniele Roche, Genevieve Almouzni, and Jean-Pierre Quivy
Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes
Kyong Hwa Moon, Xinliang Zhao, and Yi-Tao Yu
Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpoles
David Stewart, Akihiro Tomita, Yun-Bo Shi, and Jiemin Wong
Cytoplasmic mRNA Polyadenylation and Translation Assays
Maria Pique, Jose Manuel Lopez, and Raul Mendez
Xenopus Egg Extracts: A Model System to Study Proprotein Convertases
Kathleen I. J. Shennan
Analysis of Molecular Chaperones Using a Xenopus Oocyte Protein Refolding Assay
John J. Heikkila,Angelo Kaldis, and Rashid Abdulle
Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extracts
Anna Castro, Suzanne Vigneron, Cyril Bernis, Jean-Claude Labbe, and Thierry Lorca
Introduction to Nucleocytoplasmic Transport: Molecules and Mechanisms
Reiner Peters
Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studies
Reiner Peters
Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy
Bohumil Maco, Birthe Fahrenkrog, Ning-Ping Huang, and Ueli Aebi
In Vitro Study of Nuclear Assembly and Nuclear Import Using Xenopus Egg Extracts
Rene C. Chan and Douglass J. Forbes
Use of Intact Xenopus Oocytes in Nucleocytoplasmic Transport Studies
Nelly Pante
Studying the Mechanosensitivity of Voltage-Gated Channels Using Oocyte Patches
Catherine E. Morris, Peter F. Juranka, Wei Lin, Terence J. Morris, and Ulrike Laitko
Oocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and Pesticides
Steven David Buckingham, Luanda Pym, and David Barry Sattelle
Microtransplantation of Neurotransmitter Receptors From Cells to Xenopus Oocyte Membranes: New Procedure for Ion Channel Studies
Ricardo Miledi, Eleonora Palma, and Fabrizio Eusebi
Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extracts in Semi-Intact MDCK Cells
Fumi Kano, Katsuya Takenaka, and Masayuki Murata
Exploring RNA Virus Replication in Xenopus Oocytes
Andrea V. Gamarnik and Raul Andino
Study of Apoptosis in Vitro Using the Xenopus Egg Extract Reconstitution System
Paula Deming and Sally Kornbluth
Studying Fertilization in Cell-Free Extracts: Focusing on Membrane/Lipid Raft Functions and Proteomics
Ken-ichi Sato, Ken-ichi Yoshino, Alexander A. Tokmakov, Tetsushi Iwasaki, Kazuyoshi Yonezawa, and Yasuo
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