Calcium signaling protocols
著者
書誌事項
Calcium signaling protocols
(Methods in molecular biology / John M. Walker, series editor, 312)
Humana Press, c2006
2nd ed
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注記
Includes bibliographical references and index
収録内容
- Fluorescent measurement of [Ca2+]c : basic practical considerations / Alec W.M. Simpson
- Measurement of [Ca2+]i in whole cell suspensions using fura-2 / Robert A. Hirst ... [et al.]
- Measurement of [Ca2+]i in cell suspensions using indo-1 / Adriaan Nelemans
- Confocal microscopy : theory and applications for cellular signaling / Stephen C. Tovey, Paul J. Brighton, and Gary B. Willars
- Single-cell and subcellular measurement of intracellular Ca2+ concentration / Anthony J. Morgan and Andrew P. Thomas
- Ratiometric Ca2+ measurements using the FlexStation scanning fluorometer / Ian C.B. Marshall, Izzy Boyfield, and Shaun McNulty
- Measuring Ca2+ changes in multiwell format using the fluorometric imaging plate reader / Ian C.B. Marshall, Davina E. Owen, and Shaun McNulty
- Measurement of Ca2+ entry into cells using 45Ca2 / Mercedes Villarroya ... [et al.]
- Measurement of [3H]PN200-110 and [125I]ω-conotoxin MVIIA binding / Kazuyoshi Hirota and David G. Lambert
- Whole-cell patch clamp recording of voltage-sensitive Ca2+ channel currents : heterologous expression systems and dissociated brain neurons / Atticus H. Hainsworth, Andrew D. Randall, and Alessandro Stefani
- Measurement of phospholipase C by monitoring inositol phosphates using [3H]inositol-labeling protocols in permeabilized cells / Alison Skippen, Philip Swigart, and Shamshad Cockcroft
- Measurement of inositol(1,4,5)triphosphate using a stereospecific radioreceptor mass assay / Darren Smart
- Measurement of calcium fluxes in permeabilized cells using a 45Ca2+ uptake and release assay / Robert A. Wilcox
- Microinjection of myo-inositol(1,4,5)trisphosphate and other calcium-mobilizing agents into intact adherent cells / Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann
- Measurement of free [Ca2+] changes in agonist-sensitive internal stores using compartmentalized fluorescent indicators / Aldebaran M. Hofer
- Measurement of [Ca2+]i in smooth muscle strips using front-surface fluorimetry / Hideo Kanaide
- Measurement of calcium and movement in heart cells / Leong L. Ng and Paulene A. Quinn
- Simultaneous analysis of intracellular pH and Ca2+ from cell populations / Raul Martinez-Zaguilan ... [et al.]
- Measurement of cytosolic-free Ca2+ in plant tissue / Martin R. McAinsh and Carl K.-Y. Ng
- Assay and purification of calmodulin-dependent protein kinase / Rajendra K. Sharma
- Assay and purification of calmodulin-dependent cyclic nucleotide phosphodiesterase and isozyme separation / Rajendra K. Sharma
- Measurement of Ca2+-ATPase activity (in PMCA and SERCA1) / Danuta Kosk-Kosicka
内容説明・目次
内容説明
In the first edition of Calcium Signaling Protocols I began by writing "The regula- 2+ tion of intracellular Ca is a common theme presented in many papers over the last 20 2+ or so years and the description of the Ca -sensitive indicator dye fura-2 in 1985 resulted in a massive increase in these types of studies. " This statement is as true in 2005 as it was in 1999, but 20 or so years is now 30 years! There has been some reorganization of the volume such that there are now 22 ch- ters including five new ones, all written by experts in their field. These new chapters 2+ include use of the FlexStation and electrophysiological measurement of Ca channel activity. The book is broken into six parts. Part I is a general coverage of basic theory and the simplest use of fluorescent indicators. Part II covers specialist measurement 2+ systems and Part III covers measurement of Ca channel activity. Assessment of 2+ release of stored Ca is covered in some detail in Part IV, with Parts V and VI cover- 2+ ing specialist measurement techniques and Ca -sensitive targets. Putting a book like this together, even as a second edition, takes time and I am, again, indebted to the individual authors for their help and patience. I am also very grateful to Professor John M. Walker, the series editor, for his continued help and advice over the course of this project.
目次
Part I. General
Fluorescent Measurement of [Ca2+]c: Basic Practical Considerations
Alec W. M. Simpson
Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
Robert A. Hirst, Charlotte Harrison, Kazuyoshi Hirota, and David G. Lambert
Measurement of [Ca2+]i in Cell Suspensions Using Indo-1
Adriaan Nelemans
Part II. Specialist Measurement Systems
Confocal Microscopy: Theory and Applications for Cellular Signaling
Stephen C. Tovey, Paul J. Brighton, and Gary B. Willars
Single-Cell and Subcellular Measurement of Intracellular Ca2+ Concentration
Anthony J. Morgan and Andrew P. Thomas
Ratiometric Ca2+ Measurements Using the FlexStation (R) Scanning Fluorometer
Ian C. B. Marshall, Izzy Boyfield, and Shaun McNulty
Measuring Ca2+ Changes in Multiwell Format Using the Fluorometric Imaging Plate Reader
Ian C. B. Marshall, Davina E. Owen, and Shaun McNulty
Part III. Measurement of Ca2+ Channel Activity
Measurement of Ca2+ Entry Using 45Ca2+
Mercedes Villarroya, Manuela G. Lopez, Maria F. Cano-Abad, and Antonio G. Garcia
Measurement of [3H]PN200-110 and [125I]w-Conotoxin MVIIA Binding
Kazuyoshi Hirota and David G. Lambert
Whole-Cell Patch Clamp Recording of Voltage-Sensitive Ca2+ Channel Currents: Heterologous Expression Systems and Dissociated Brain Neurons
Atticus H. Hainsworth, Andrew D. Randall, and Alessandro Stefani
Part IV. Measurement of Ins(1,4,5)P3 and Ca2+ Release From Intracellular Stores
Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protocols in Permeabilized Cells
Alison Skippen, Philip Swigart, and Shamshad Cockcroft
Measurement of Inositol(1,4,5)Triphosphate Using a Stereospecific Radioreceptor Mass Assay
Darren Smart
Measurement of Calcium Fluxes in Permeabilized Cells Using a45Ca2+ Uptake and Release Assay
Robert A. Wilcox
Microinjection of myo-Inositol(1,4,5)Trisphosphate and Other Calcium-Mobilizing Agents Into Intact Adherent Cells
Robert A. Wilcox, Ian D. Forsythe, and Terence J. McCann
Measurement of Free [Ca2+] Changes in Agonist-Sensitive Internal Stores Using Compartmentalized Fluorescent Indicators
Aldebaran M. Hofer
Part V. Specialist Measurement Techniques
Measurement of [Ca2+]i in Smooth Muscle Strips Using Front-Surface Fluorimetry
Hideo Kanaide
Measurement of Calcium and Movement in Heart Cells
Leong L. Ng and Paulene A. Quinn
Simultaneous Analysis of Intracellular pH and Ca2+ From Cell Populations
Raul Martinez-Zaguilan, Linda S. Tompkins, Robert J. Gillies, and Ronald M. Lynch
Measurement of Cytosolic-Free Ca2+ in Plant Tissue
Martin R. McAinsh and Carl K.-Y. Ng
Part VI. Ca2+-Sensitive Targets
Assay and Purification of Calmodulin-Dependent Protein Kinase
Rajendra K. Sharma
Assay and Purification of Calmodulin-Dependent Cyclic Nucleotide Phosphodiesterase and Isozyme Separation
Rajendra K. Sharma
Measurement of Ca2+-ATPase Activity (in PMCA and SERCA1)
Danuta Kosk-Kosicka
Index
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