Hormone assays in biological fluids

Author(s)

Bibliographic Information

Hormone assays in biological fluids

edited by Michael J. Wheeler, J.S. Morley Hutchinson

(Methods in molecular biology / John M. Walker, series editor, 324)

Humana Press, c2006

Available at  / 4 libraries

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Note

Includes bibliographical references and index

Contents of Works

  • Immunoassay techniques / Michael J. Wheeler
  • High-performance liquid chromatography for hormone assay / John W. Honour
  • Gas chromatography-mass spectrometry / John W. Honour
  • An introduction to PCR techniques / Gill Rumsby
  • Methods for the investigation of thyroid function / Christine R. Squire
  • Assays for LH, FSH, and prolactin / Michael J. Wheeler
  • Adrenocorticotropic hormone and related peptides / Les A. Perry
  • Measurement of glucocorticoids in biological fluids / Geoff Holder
  • Urinary steroid profiling / Norman F. Taylor
  • Measurement of aldosterone in blood / Marion L. Cawood
  • Measurement of plasma renin activity / Marion L. Cawood
  • Measurement of androgens / Michael J. Wheeler
  • Measurement of gut hormones in plasma / Shahred Taheri, Mohammed Ghatei and Steve Bloom
  • Measurement of melatonin and 6-sulphatoxymelatonin / Benita Middleton

Description and Table of Contents

Description

Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.

Table of Contents

Immunoassay Techniques Michael J. Wheeler High-Performance Liquid Chromatography for Hormone Assay John W. Honour Gas Chromatography-Mass Spectrometry John W. Honour An Introduction to PCR Techniques Gill Rumsby Methods for the Investigation of Thyroid Function Christine R. Squire Assays for LH, FSH, and Prolactin Michael J. Wheeler Adrenocorticotropic Hormone and Related Peptides Les A. Perry Measurement of Glucocorticoids in Biological Fluids Geoff Holder Urinary Steroid Profiling Norman F. Taylor Measurement of Aldosterone in Blood Marion L. Cawood Measurement of Plasma Renin Activity Marion L. Cawood Measurement of Androgens Michael J. Wheeler Measurement of Gut Hormones in Plasma Shahrad Taheri, Mohammad Ghatei, and Steve Bloom Measurement of Melatonin and 6-Sulphatoxymelatonin Benita Middleton Index

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