The condensed protocols from molecular cloning : a laboratory manual

Bibliographic Information

The condensed protocols from molecular cloning : a laboratory manual

Joseph Sambrook, David W. Russell

Cold Spring Harbor Laboratory Press, c2006

  • : hardcover
  • : pbk

Available at  / 32 libraries

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Note

Includes index

Contents of Works

  • Plasmids and their usefulness in molecular cloning
  • Bacteriophage λ and its vectors
  • Working with bacteriophage M13 vectors
  • Working with high-capacity vectors
  • Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis
  • Preparation and analysis of eukaryotic genomic DNA
  • Extraction, purification, and analysis of mRNA from eukaryotic cells
  • In vitro amplification of DNA by the polymerase chain reaction
  • Preparation of radiolabeled DNA and RNA probes
  • Working with synthetic oligonucleotide probes
  • Preparation of cDNA libraries and gene identification
  • DNA sequencing
  • Mutagenesis
  • Screening expression libraries
  • Expression of cloned genes in escherichia coli
  • Introducing cloned genes into cultured mammalian cells
  • Analysis of gene expression in mammalian cells
  • Protein interaction technologies

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