The condensed protocols from molecular cloning : a laboratory manual
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書誌事項
The condensed protocols from molecular cloning : a laboratory manual
Cold Spring Harbor Laboratory Press, c2006
- : hardcover
- : pbk
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注記
Includes index
収録内容
- Plasmids and their usefulness in molecular cloning
- Bacteriophage λ and its vectors
- Working with bacteriophage M13 vectors
- Working with high-capacity vectors
- Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis
- Preparation and analysis of eukaryotic genomic DNA
- Extraction, purification, and analysis of mRNA from eukaryotic cells
- In vitro amplification of DNA by the polymerase chain reaction
- Preparation of radiolabeled DNA and RNA probes
- Working with synthetic oligonucleotide probes
- Preparation of cDNA libraries and gene identification
- DNA sequencing
- Mutagenesis
- Screening expression libraries
- Expression of cloned genes in escherichia coli
- Introducing cloned genes into cultured mammalian cells
- Analysis of gene expression in mammalian cells
- Protein interaction technologies