MicroRNA protocols
著者
書誌事項
MicroRNA protocols
(Methods in molecular biology / John M. Walker, series editor, v. 342)
Humana Press, c2006
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.
目次
The MicroRNA: Overview of the RNA Gene That Modulates Gene Functions
Shao-Yao Ying, Donald C. Chang, Joseph D. Miller, and Shi-Lung Lin
Structure Analysis of MicroRNA Precursors
Jacek Krol and Wlodzimierz J. Krzyzosiak
MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex
Richard I. Gregory, Thimmaiah P. Chendrimada, and Ramin Shiekhattar
Recognition and Cleavage of Primary MicroRNA Transcripts
Yan Zeng and Bryan R. Cullen
Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery
Stefan A. Muljo and Chryssa Kanellopoulou
MicroRNAs and Messenger RNA Turnover
Julia A. Chekanova and Dmitry A. Belostotsky
Prediction of MicroRNA Targets
Marc Rehmsmeier
Prediction of Human MicroRNA Targets
Bino John, Chris Sander, and Debora S. Marks
Complications in Mammalian MicroRNA Target Prediction
Neil R. Smalheiser and Vetle I. Torvik
miRBase:The MicroRNA Sequence Database
Sam Griffiths-Jones
Methodologies for High-Throughput Expression Profiling of MicroRNAs
Paz Einat
In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development
Catherine Kidner and Marja Timmermans
Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA
Fengfeng Zhuang and Yi-Hsin Liu
Cloning MicroRNAs From Mammalian Tissues
Michael Z. Michael
Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation
Hyeyoung Min and Chang-Zheng Chen
Identifying MicroRNA Regulators of Cell Death in Drosophila
Chun-Hong Chen, Ming Guo, and Bruce A. Hay
MicroRNAs in Human Immunodeficiency Virus-1 Infection
Yamina Bennasser, Shu-Yun Le, Man Lung Yeung, and Kuan-Teh Jeang
Cloning and Detection of HIV-1-EncodedMicroRNA
Shinya Omoto and Yoichi R. Fujii
Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein
Ranhui Duan and Peng Jin
In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates
Akira Ishizuka, Kuniaki Saito, Mikiko C. Siomi, and Haruhiko Siomi
Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector
Feng Luo, Jiing-Kuan Yee, Sheng-He Huang, Ling-Tao Wu, and Ambrose Y. Jong
Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs
Shi-Lung Lin and Shao-Yao Ying
Isolation and Identification of Gene-Specific MicroRNAs
Shi-Lung Lin, Donald C. Chang, and Shao-Yao Ying
Transgene-Like Animal Models Using Intronic MicroRNAs
Shi-Lung Lin, Shin-Ju E. Chang, and Shao-Yao Ying
Evolution of MicroRNAs
Andrea Tanzer and Peter F. Stadler
Perspectives
Shao-Yao Ying, Donald C. Chang, Joseph D. Miller, and Shi-Lung Lin
Index
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