Dictyostelium discoideum protocols

著者

• Eichinger, Ludwig
• Rivero-Crespo, Francisco

書誌事項

Dictyostelium discoideum protocols

edited by Ludwig Eichinger, Francisco Rivero

（Methods in molecular biology / John M. Walker, series editor, v. 346）

Humana Press, c2006

• : [pbk.]

注記

Includes bibliographical references and index

内容説明・目次

内容説明

Dictyostelium discoideum is a simple but fascinating eukaryotic microorg- ism, whose natural habitat is deciduous forest soil and decaying leaves, where the amoebae feed on bacteria and grow as independent single cells. Exhaustion of the bacterial food source triggers a developmental program, in which up to 100,000 cells aggregate by chemotaxis towards cAMP. Morphogenesis and cell different- tion then culminate in the production of spores enabling the organism to survive unfavorable conditions. Dictyostelium offers unique advantages for studying f- damental cellular processes with the aid of powerful molecular genetic, bioche- cal, and cell biological tools. These processes include signal transduction, chemotaxis, cell motility, cytokinesis, phagocytosis, and aspects of development such as cell sorting, pattern formation and cell type differentiation. Recently, D- tyostelium was also described as a suitable host for pathogenic bacteria in which one can conveniently study the process of infection. In addition, Dictyostelium has many of the experimental conveniences of Saccharomyces cerevisiae and is pr- ably the best experimentally manipulatable protozoan, providing insight into this diverse group of organisms, which includes some of the most dangerous human parasites. The recent completion of the Dictyostelium genome sequencing project strengthens the position of D. discoideum as a model organism. The completed genome sequence and other valuable community resources constitute the source for basic biological and biomedical research and for genome-wide analyses.

目次

Part I. The Organism, Community Resources, and Genome-wide Studies The Secret Lives of Dictyostelium Richard H. Kessin The Genome of Dictyostelium discoideum Adam Kuspa and William F. Loomis The cDNA Sequencing Project Hideko Urushihara, Takahiro Morio, and Yoshimasa Tanaka dictyBase and the Dicty Stock Center Petra Fey, Pascale Gaudet, Karen E. Pilcher, Jakob Franke, and Rex L. Chisholm Analysis of Gene Expression Using cDNA Microarrays Marcel Kaul and Ludwig Eichinger Proteomic Analysis of Dictyostelium discoideum Udo Roth, Stefan Muller, and Franz-Georg Hanisch Part II. Basic Methods and Molecular Genetic Techniques Cultivation, Spore Production, and Mating Hideko Urushihara Parasexual Genetics Using Axenic Cells Jason King and Robert Insall Slug Phototaxis, Thermotaxis, and Spontaneous Turning Behavior Paul R. Fisher and Sarah J. Annesley Purification Techniques of Subcellular Compartments for Analytical and Preparative Purposes Laurence Aubry and Gerard Klein Generation of Multiple Knockout Mutants Using the Cre-loxP System Alan R. Kimmel and Jan Faix Restriction Enzyme-Mediated Integration (REMI) Mutagenesis Adam Kuspa RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium Markus Kuhlmann, Blagovesta Popova, and Wolfgang Nellen Part III. Imaging and Localization Methods Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies Annette Muller-Taubenberger Investigating Gene Expression: In Situ Hybridization and Reporter Genes Ricardo Escalante and Leandro Sastre Application of 2D and 3D DIAS to Motion Analysis of Live Cells in Transmission and Confocal Microscopy Imaging Deborah Wessels, Spencer Kuhl, and David R. Soll UsingQuantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Events in Single Living Cells Xuehua Xu, Joseph A. Brzostowski, and Tian Jin Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development Dirk Dormann and Cornelis J. Weijer Under-Agarose Chemotaxis of Dictyostelium discoideum David Woznica and David A. Knecht Optimized Fixation and Immunofluorescence Staining Methods for Dictyostelium Cells Monica Hagedorn, Eva M. Neuhaus, and Thierry Soldati Cryofixation Methods for Ultrastructural Studies of Dictyostelium discoideum Mark J. Grimson and Richard L. Blanton Part IV. Dictyostelium as Model Organism Analysis of Signal Transduction: Formation of cAMP, cGMP, and Ins(1,4,5)P3 In Vivo and In Vitro Peter J. M. Van Haastert Assaying Chemotaxis of Dictyostelium Cells Michelle C. Mendoza and Richard A. Firtel Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized Light Scattering Ruth Furukawa and Marcus Fechheimer Quantitative and Microscopic Methods for Studying the Endocytic Pathway Francisco Rivero and Markus Maniak Preparation of Intact, Highly Purified Phagosomes from Dictyostelium Daniel Gotthardt, Regis Dieckmann, Vincent Blancheteau, Claudia Kistler, Frank Reichardt, and Thierry Soldati Assaying Cell-Cell Adhesion Salvatore Bozzaro Periodic Activation of ERK2 and Partial Involvement of G Protein in ERK2 Activation by cAMP in Dictyostelium Cells Mineko Maeda An Improved Method for Dictyostelium Centrosome Isolation Irene Schulz, Yvonne Reinders, Albert Sickmann, and Ralph Graf Epigenetics in Dictyostelium Markus Kaller, Wolfgang Nellen, and Jonathan R. Chubb Dictyostelium discoideum as a Model to Study

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詳細情報

• NII書誌ID(NCID): BA78591135
• ISBN:
  • 9781588296238
  • 9781617376771
• LCCN: 2005033191
• 出版国コード: us
• タイトル言語コード: eng
• 本文言語コード: eng
• 出版地: Totowa, N.J.
• ページ数/冊数: xv, 564 p.
• 大きさ: 24 cm
• 分類:
  • LCC : QK635.D5
  • NLM : W1
  • NLM : QX 25
  • DC22 : 579.5/2
• 件名:
  • LCSH : Dictyostelium discoideum -- Laboratory manuals
  • MESH : Dictyostelium -- cytology -- Laboratory Manuals
  • MESH : Dictyostelium -- genetics -- Laboratory Manuals
  • MESH : Genetic Techniques -- Laboratory Manuals
  • MESH : Models, Biological -- Laboratory Manuals
  • MESH : Models, Genetic -- Laboratory Manuals
• 親書誌ID:
  • BA10794673