Biochemistry laboratory : modern theory and techniques
著者
書誌事項
Biochemistry laboratory : modern theory and techniques
Benjamin Cummings, c2006
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Many biochemistry lab instructors are now opting to either design their own experiments or select them from major educational journals.Biochemistry Laboratory: Modern Theory and Techniques addresses this issue by providing a flexible alternative without experimental protocols. Instead of requiring instructors to use specific experiments, the book focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. The extensive range of techniques includes internet databases, chromatography, electrophoresis, spectroscopy, measurements of ligand-binding interactions, and recombinant DNA techniques such as molecular cloning and PCR.
目次
Part I: Theory and Experimental Techniques
Introduction to the Biochemistry Laboratory
The Computer as a Tool in Biochemistry and Molecular Biology
General Laboratory Procedures
Centrifugation Techniques in Biochemistry
Purification and Identification of Biomolecules by Chromatography
Characterization of Proteins and Nucleic Acids by Electrophoresis
Spectroscopic Analysis of Biomolecules
Biomolecular Interactions: Ligand Binding and Enzyme Reactions
Molecular Biology I: Structures and Analysis of Nucleic Acids
Molecular Biology II: Recombinant DNA. Molecular Cloning, and Enzymology
Protein Production, Purification, and Characterization
Part II: Teaching the Biochemistry/Molecular Biology Lab
A Brief History
A Variety of Teaching Methods
Essential BMB Concepts and Skills for Student Learning
Experiments in Biochemistry and Molecular Biology
Further Reading
Appendices.
Index.
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