Biophysical labeling methods in molecular biology
著者
書誌事項
Biophysical labeling methods in molecular biology
Cambridge University Press, 2005
- pbk.
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注記
Includes bibliographical references and Index
内容説明・目次
内容説明
Physical labels such as stable nitroxide radicals, luminescent and photochromic chromophores, so-called Moessbauer atoms and electron-dense assemblies of heavy atoms have proved to be effective tools in solving many problems at the molecular level in biological systems. These physical labels are used as 'molecular rulers' to measure the distances between chosen groups and to measure the size, form and microrelief of objects. By providing information about these factors, the label provides information that can help the scientist to understand the structure of membranes, nucleic acids, enzymes and proteins and how they function. This 1993 volume covers all aspects of this field: the theoretical bases, the experimental techniques, and it also shows how to interpret the resulting data. It also critically discusses some recent results obtained with these techniques and gives an analysis of likely developments in the future.
目次
- 1. The methods of spin labeling
- 2. Double labeling techniques
- 3. Fluorescent labeling methods
- 4. Triplet labeling methods
- 5. Moessbauer, electron-scattering, and other labeling methods
- 6. Studies of proteins and enzymes: structure, dynamics and mechanism of action
- 7. Structure and dynamics of membrane
- 8. Nucleic acids and other biosystems, biological assays
- 9. Conclusion
- References
- Subject index.
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