Animal cell biotechnology : methods and protocols

著者

    • Pörtner, Ralf

書誌事項

Animal cell biotechnology : methods and protocols

edited by Ralf Pörtner

(Methods in biotechnology, 24)

Humana Press, c2007

2nd ed.

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

"Animal Cell Biotechnology: Methods and Protocols" covers new techniques for setting up mammalian cell lines for production of biopharmaceuticals and optimizing critical parameters for cell culture considering the whole cascade from the lab to the final production. This volume is divided into six parts, each reflecting the processes required for different stages of production.

目次

  • Silvia Carroll, Mariam Nasiri and Mohamed Al-Rubeai
  • 10 Nuclear Magnetic Resonance Methods for Monitoring Cell Growth and Metabolism in Intensive Bioreactors
  • Andre A. Neves and Kevin M. Brindle
  • 11 Methods for off-line analysis of nutrients and products in mammalian cell culture
  • Heino Buntemeyer
  • 12 Application of Stoichiometric and Kinetic Analyses to Characterize Cell Growth and Product Formation
  • Derek Adams, Rashmi Korke, and Wei-Shou Hu
  • 13 Measurement of apoptosis in cell culture
  • Adiba Ishaque 1 and Mohamed Al-Rubeai
  • 14 Metabolic Flux Estimation in Mammalian Cell Cultures
  • Chetan T. Goudar, Richard Biener, James M. Piret and Konstantin B. Konstantinov
  • Part IV. Special cultivation techniques
  • 15 Disposable bioreactors for inoculum production and protein expression
  • Regine Eibl and Dieter Eibl
  • 16 Hollow Fibre Cell Culture
  • John M. Davis
  • 17 Cultivation of mammalian cells in fixed bed reactors
  • Ralf Portner and Oscar B.J. Platas Barradas
  • 18 Configuration of Bioreactors
  • Dirk E. Martens and Evert Jan van den End
  • Part V. Downstream techniques
  • 19 Membrane filtration in animal cell culture
  • Peter Czermak, Dirk Nehring and Ranil Wickramasinghe
  • 20 Chromatographic techniques in the downstream processing of (recombinant) proteins
  • Ruth Freitag.

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