Pichia protocols
著者
書誌事項
Pichia protocols
（Methods in molecular biology / John M. Walker, series editor, v. 389）
Humana Press, c2007
2nd ed.
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.
目次
Table of Contents
Chapter 1 - Introduction [REVIEW CHAPTER]
Chapter 2 - Vectors and strains for expression [REVIEW CHAPTER]
Chapter 3 - DNA-Mediated Transformation
Chapter 4 - Rational design and optimization of fed-batch and continuous fermentations
Chapter 5 - Overexpression of a hookworm secretory protein saturates secretory capacity
Chapter 6 - Initial processing and purification strategies
Chapter 7 - Rapid screening of chromatography resins for the purification of proteins
Chapter 8 - Characterization of O-linked saccharides on glycoproteins
Chapter 9 - Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using GlycoSwitch plasmids
Chapter 10 - N-linked glycan characterization of heterologous proteins
Chapter 11 - Heavy labeling of recombinant proteins
Chapter 12 - Selenomethionine labeling of recombinant proteins
Chapter 13 - Deuterium labeling of proteins
Chapter 14 - Classical genetic analysis
Chapter 15 - Identification of pexophagy genes by Restriction Enzyme-Mediated Integration (REMI)
Chapter 16 - Characterization of protein-protein interactions
Chapter 17 - Localization of proteins and organelles using fluorescence microscopy
Chapter 18 - Fluorescence microscopy and thin-section electron microscopy
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