Pichia protocols
著者
書誌事項
Pichia protocols
（Methods in molecular biology / John M. Walker, series editor, v. 389）
Humana Press, c2007
2nd ed.
大学図書館所蔵 全8件
注記
Includes bibliographical references and index
内容説明・目次
内容説明
This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.
目次
Table of Contents
Chapter 1 - Introduction [REVIEW CHAPTER]
Chapter 2 - Vectors and strains for expression [REVIEW CHAPTER]
Chapter 3 - DNA-Mediated Transformation
Chapter 4 - Rational design and optimization of fed-batch and continuous fermentations
Chapter 5 - Overexpression of a hookworm secretory protein saturates secretory capacity
Chapter 6 - Initial processing and purification strategies
Chapter 7 - Rapid screening of chromatography resins for the purification of proteins
Chapter 8 - Characterization of O-linked saccharides on glycoproteins
Chapter 9 - Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using GlycoSwitch plasmids
Chapter 10 - N-linked glycan characterization of heterologous proteins
Chapter 11 - Heavy labeling of recombinant proteins
Chapter 12 - Selenomethionine labeling of recombinant proteins
Chapter 13 - Deuterium labeling of proteins
Chapter 14 - Classical genetic analysis
Chapter 15 - Identification of pexophagy genes by Restriction Enzyme-Mediated Integration (REMI)
Chapter 16 - Characterization of protein-protein interactions
Chapter 17 - Localization of proteins and organelles using fluorescence microscopy
Chapter 18 - Fluorescence microscopy and thin-section electron microscopy
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