Genetic variation : a laboratory manual

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書誌事項

Genetic variation : a laboratory manual

edited by Michael P. Weiner, Stacey B. Gabriel, J. Claiborne Stephens

Cold Spring Harbor Laboratory Press, c2007

  • pbk.
  • hard

大学図書館所蔵 件 / 全16件

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注記

Includes bibliographical references and index

内容説明・目次

内容説明

Genetic Variation: A Laboratory Manual is the first compendium of protocols specifically geared towards genetic variation studies, and includes thorough discussions on their applications for human and model organism studies. Intended for graduate students and professional scientists in clinical and research settings, it covers the complete spectrum of genetic variation-from SNPs and microsatellites to more complex DNA alterations, including copy number variation. Written and edited by leading scientists in the field, the early sections of the manual are devoted to study design and generating genotype data, the use of resources such as HapMap and dbSNP, as well as experimental, statistical, and bioinformatic approaches for analyzing the data. The final sections include descriptions of genetic variation in model organisms and discussions of recent insights into human genetic ancestry, forensics, and human variation.

目次

  • 1. Ethical Issues in Human Genetic Research: The Global Experience K. Arnold and J. van der Walt SECTION 1: STUDY DESIGN Introduction 2. Population Choice as a Consideration for Genetic Analysis Study Design J.C. Stephens and M. Bamshad 3. Power Calculations D.M. Evans and S. Purcell 4. Genetic Anaylsis: Moving between Linkage and Association A.V. Smith 5. NCBI dbSNP Database: Content and Searching M.L. Feolo and S.T. Sherry 6. Using the HapMap Web Site A.V. Smith SECTION 2: LABORATORY PROTOCOLS Introduction PART 1: PREPARATION OF DNA AND RNA 7. Isolation of Plant DNA for Genotyping Analysis N.M. Springer 8. Preparing RNA from Plant Tissues A.-P. Hsia, H.D. Chen, K. Ohtsu, and P.S. Schnable 9. Preparing DNA from Mammalian Sources A. Sahota, A.I. Brooks, and J.A. Tischfield PART 2: SNP VARIATION ANALYSIS 10. Intermediate-Throughput Laboratory-Scale Genotyping Solutions S.J. Macdonald 11. Intermediate-Throughput Laboratory-Scale Genotyping Protocols E. Cuppen, S.J. Macdonald, C. Ha, P.-Y. Kwok, W.B. Barbazuk, A.-P. Hsia, H.D. Chen, Y. Fu, K. Ohtsu, and P.S. Schnable 12. Molecular Inversion Probes and Universal Tag Arrays: Application to Highplex Targeted SNP Genotyping G. Karlin-Neumann, M. Sedova, R. Sapolsky, J. Forman, Y. Wang, M. Moorhead, and M.Faham 13. Whole-Genome Genotyping S.B. Gabriel and M.P. Weiner PART 3: COPY NUMBER AND COMPLEX VARIATION ANALYSIS 14. Comparative Genomic Hybridization to Detect Variation in the Copy Number of Large DNA Segments I.N. Holcomb and B.J. Trask 15. Representational Oligonucleotide Microarray Analysis Detection of Genetic Variation R. Lucito 16. Whole-Genome Sampling Analysis to Detect Copy Number Changes in FFPE Samples S. Jacobs 17. Molecular Inversion Probe Targeted Genotyping: Application to Copy Number Determination G.Karlin-Neumann, M. Sedova, R. Sapolsky, S. Lin, Y. Wang, M. Moorhead, and M. Faham 18. Microsatellite Markers for Linkage and Association Studies J. Gulcher SECTION 3: DATA ANALYSIS Introduction 19. Considerations for SNP Selection C. Carlson 20. Selection and Evaluation of tag-SNPs Using Tagger and HapMap P. de Bakker 21. Haploview: Visualization and Analysis of SNP Genotype Data J.C. Barrett 22. Considerations for Copy Number Analysis of FFPE Samples S. Jacobs 23. Assessing Significance in Genetic Association Studies M.J. Daly 24. Assessing Human Variation Data for Signatures of Natural Selection M. Bamshad and J.C. Stephens SECTION 4: VARIATION STUDIES IN MODEL ORGANISMS Introduction 25. Arabidopsis Y. Li and J.O. Borevitz 26. Maize W.B. Barbazuk, A.-P. Hsia, H.D. Chen, Y. Fu, K. Ohtsu, and P.S. Schnable 27. Rice H. Leung, K.L. McNally, and D. Mackill 28. The Mouse C.M. Wade and M.J. Daly 29. The Rat E. Cuppen, N. H fc
  • bner, H.J. Jacob, and A.E. Kwitek 30. The Cat M.J. Lipinski, N. Billings, L.A. Lyons 31. The Dog K. Lindblad-Toh and E.A. Ostrander 32. The Chimpanzee T.S. Mikkelsen, M.C. Zody, and K. Lindblad-Toh SECTION 5: INSIGHTS INTO HUMAN VARIATION Introduction 33. Genealogical Markers: mtDNA and the Y Chromosome M. Stoneking and M. Kayser 34. Forensic DNA Testing J.M. Butler 35. The Human Genome: What Lies Ahead M.P. Weiner and J.C. Stephens APPENDIX: Cautions INDEX

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