Patch-clamp methods and protocols
著者
書誌事項
Patch-clamp methods and protocols
(Methods in molecular biology / John M. Walker, series editor, 403)
Humana Press, c2007
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注記
Includes bibliographical references and index
内容説明・目次
内容説明
Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively.
The volume includes an extensive theoretical treatise concerning single channel kinetic analysis.
目次
Table of contents
Part 1. Pharmacology
1. Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDA
receptors using whole-cell patch clamp method. Laszlo Fodor and Jozsef
Nagy
2. Memantine as an example of a fast, voltage-dependent, open channel
N-methyl-D-aspartate (NMDA) receptor blocker. Chris G. Parsons and Kate
Gilling
3. Methods for evaluation of positive allosteric modulators of
glutamate AMPA receptors. Edward R. Siuda, Jennifer C. Quirk and Eric
S. Nisenbaum
4. Automated voltage clamp technique. Andrea Ghetti, Antonio Guia and
Jia Xu
5. Flip-the-Tip: automated patch clamping based on glass electrodes.
Michael Fejtl, Uwe Czubayko, Alexander Hummer, Tobias Krauter and
Albrecht Lepple-Wienhues
6. The Roboocyte: Automated Electrophysiology Based on Xenopus Oocytes.
Christine Leisgen, Mike Kuester and Christoph Methfessel
Part 2. Physiology
7. Infrared guided laser stimulation as a tool for elucidating the
synaptic site of expression of long-term synaptic plasticity. Gerhard
Rammes, Mathias Eder, Walter Zieglgansberger and Hans-Ulrich Dodt
8. Single-cell RT-PCR, a Technique to Decipher the Electrical,
Anatomical and Genetic Determinants of Neuronal Diversity. Maria
Toledo-Rodriguez and Henry Markram
9. Mechanosensitive Ion Channels investigated simultaneously by
Scanning Probe Microscopy and Patch clamp. Matthias G. Langer
10. Synaptic Connectivity in Engineered Neuronal Networks. Peter
Molnar, Jung-Fong Kang, Neelima Bhargava, Mainak Das and James J.
Hickman
11. Modeling of Action Potential Generation in NG108-15 Cells. Peter
Molnar and James J. Hickman
12. Whole-cell Voltage-clamp on Skeletal Muscle Fibers with the
Silicone-Clamp Technique. Sandrine Pouvreau, Claude Collet, Bruno
Allard and Vincent Jacquemond
13. Determination of channel properties at the unitary level in adult
mammalian isolated cardiomyocytes. RomainGuinamard
14. Electrophysiological Properties of Embryonic Stem Cells During
Differentiation into Cardiomyocyte-like Cell Types. Antoni C.G. van
Ginneken and Arnoud. C. Fijnvandraat
15. Hybrid Neuronal Network Studies Under Dynamic Clamp. Alan D. Dorval
II, Jonathan Bettencourt, Theoden I. Netoff and John A. White
16. Cardiac channelopathies studied with the dynamic action potential
clamp technique. Geza Berecki, Jan G. Zegers, Ronald Wilders and Antoni
C.G. van Ginneken
Part 3. Biophysics
17. Principles of Single-Channel Kinetic Analysis. Feng Qin *
Theoretical chapter
18. Use of Xenopus oocytes to measure ionic selectivity of pore-forming
peptides and ion channels. Thierry Cens and Pierre Charnet
19. Estimation of quantal parameters with multiple-probability
fluctuation analysis. Chiara Saviane and Robin Angus Silver
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