Germline stem cells
Author(s)
Bibliographic Information
Germline stem cells
(Methods in molecular biology / John M. Walker, series editor, v. 450)
Humana Press, c2008
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Note
Includes bibliographical references and index
"e-ISBN:9781603272148"--t. p. verso
Description and Table of Contents
Description
In this comprehensive and cutting-edge book, leading experts explore the parameters that define germline stem cells and the mechanisms that regulate the cell behavior in order to better isolate, characterize and maintain them. The volume begins by providing protocols for germline stem cell identification and regulation in model organisms, and concludes with detailed chapters covering current techniques involving in vitro culture and the applications of the cells.
Table of Contents
Contents Preface............................................................................................ Contributors...................................................................................... Part I Identification and Regulation of Germline Stem Cells in Model Organisms The Development of Germline Stem Cells in Drosophila David A. Dansereau and Paul Lasko................................................... Analysis of the C. elegans Germline Stem Cell Region Sarah L. Crittenden and Judith Kimble .............................................. Immunohistological Techniques for Studying the Drosophila Male Germline Stem Cell Shree Ram Singh and Steven X. Hou................................................. Genetic Tools Used for Cell Lineage Tracing and Gene Manipulation in Drosophila Germline Stem Cells Wei Liu and Steven X. Hou............................................................ Structural Polarity and Dynamics of Male Germline Stem Cells in an Insect (the milkweed bug Oncopeltus fasciatus) David C. Dorn and August Dorn...................................................... High Resolution Light Microscopic Characterization of Spermatogonia Helio Chiarini-Garcia and Marvin L Meistrich...................................... Identification and Characterization of Spermatogonial Subtypes and Their Expansion in Whole Mounts and Tissue Sections from Primate Testes Jens Ehmcke and Stefan Schlatt........................................................................... Epigenetic Control in Male Germ Cells Durga Prasad Mishra and PaoloSassone-Corsi..................................... GDNF Maintains Mouse Spermatogonial Stem Cells in vivo and in vitro Hannu Sariola and Tiina Immonen..................................................... Part II In vitro Culture and Applications of Germline Stem Cells Ectopic Grafting of Mammalian Testis Tissue into Mouse Hosts Ina Dobrinski and Rahul Rathi............................................. Spermatogonial Stem Cell Transplantation, Testicular Function and Restoration of Male Fertility in Mice Derek J. McLean.............................................................................. 12 Isolating Highly Pure Rat Spermatogonial Stem Cells in Culture F. Kent Hamra, Karen M. Chapman, Zhuoru Wu, and David L. Garbers...chrically Complex Trait Jason D. Heaney and Joseph H. Nadeau................................................ Study Origin of Germ Cells and Formation of New Primary Follicles in Adult Human and Rat Ovaries Antonin Bukovsky, Satish K. Gupta, Irma Virant-Klun, Nirmala B. Upadhyaya, Pleas Copas, Stuart E. Van Meter, Marta Svetlikova, Maria E. Ayala, and Roberto Dominguez .............................................................. Index.....................................................................................................
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