Food-borne pathogens : methods and protocols
Author(s)
Bibliographic Information
Food-borne pathogens : methods and protocols
(Methods in biotechnology, 21)
Humana Press, c2006
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Note
Includes bibliographical references and index
Description and Table of Contents
Description
A collection of readily reproducible classic and emerging molecular methods for the laboratory isolation and identification of the pathogens, viruses, and parasites that cause food-borne disease. Among the pathogens covered are specific bacteria, including Salmonella spp, Campylobacter spp., Listeria spp., and Bacillus spp.; viruses, including noroviruses and enteroviruses; and parasites, including Cryptosporidium and seafood nematode worms. The protocols follow the successful Methods in Biotechnology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Table of Contents
Part I. The Bacteria
Detection of Hemolysins in Aeromonas spp. Isolates From Food Sources: PCR Analysis and Biological Activity
Rosabel Falcon, Tatiana d'Albuquerque e Castro, Maria das Gracas de Luna, Angela Correa de Freitas-Almeida, and Tomomasa Yano
Detection and Purification of Bacillus cereus Enterotoxins
Toril Lindback and Per Einar Granum
Campylobacter: Isolation, Identification, and Preservation
Rachel Gorman and Catherine C. Adley
Detection of Clostridium botulinum by Multiplex PCR in Foods and Feces
Miia Lindstroem, Mari Nevas, and Hannu Korkeala
Multiplex PCR for Specific Identification of Enterohemorrhagic Escherichia coli Strains in the O157:H7 Complex
Peter C. H. Feng and Steven R. Monday
PulseNet's Step-by-Step Laboratory Protocol for Molecular Subtyping of Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis
Lewis M. Graves and Balasubramanian Swaminathan
Plesiomonas shigelloides: Detection by PCR
Ivan Ciznar, Carlos Gonzalez-Rey, Karel Krovacek, and Anna Hostacka
Pulsed-Field Gel Electrophoresis As a Molecular Technique in Salmonella Epidemiological Studies
Rachel Gorman and Catherine C. Adley
Kits for Detection of Food Poisoning Toxins Produced by Bacillus cereus and Staphylococcus aureus
Moira M. Brett
Microbiological and Molecular Methods to Identify and Characterize Toxigenic Vibrio cholerae From Food Samples
Keya De, Ranjan K. Nandy, and G. Balakrish Nair
HPLC Measurement of Aflatoxin B1 and Metabolites in Isolated Rat Hepatocytes
Jennifer Colford
Part II. The Viruses
Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR
Christian M. Beuret
Detection of Enteroviruses
Miguel-Angel Jimenez-Clavero, Victoria Ley, Nuria Gomez, and Juan-CarlosSaiz
Detection of Hepatitis A Virus and Rotavirus Using Nucleic Acid Sequence-Based Amplification
Julie Jean and Ismail Fliss
Part III. The Parasites
Isolation and Characterization of Cathepsin-L1 Protease From Fasciola hepatica Excretory-Secretory Products for Serodiagnosis of Human Fasciolosis
Sandra M. O'Neill, Grace Mulcahy, and John P. Dalton
Molecular Biology Methods for Detection and Identification of Cryptosporidium Species in Feces, Water, and Shellfish
Colm J. Lowery, L. Xiao, U. M. Ryan, James S. G. Dooley, B. Cherie Millar, and John E. Moore
Molecular Identification of Nematode Worms From Seafood (Anisakis spp. and Pseudoterranova spp.) and Meat (Trichinella spp.)
Giuseppe La Rosa, Stefano D'Amelio, and Edoardo Pozio
Part IV. Parallel Studies to the Analysis of Food-Borne Pathogens
Approaches to Developing Quantitative Risk Assessment Models
Enda J. Cummins
A Review of Surveillance Networks of Food-Borne Diseases
Camelia Molnar, Rita Wels, and Catherine C. Adley
Index
by "Nielsen BookData"